黄芩苷对人肺腺癌A549细胞的体内外研究  

Effect of Baicalin on Human Lung Cancer A549 Cell Line

作　　者：曹慧娟[1] 鄢云彪 戴建业[1] 孙淑军[1] 郑宁宁[1] 房军伟[1] 王洋[1] 李倩华[1] 张永煜[1] 

机构地区：[1]上海中医药大学中医方证与系统生物学研究中心,上海201203 [2]上海药明康德新药开发有限公司,上海200131

出　　处：《中国实验方剂学杂志》2013年第13期216-220,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：上海市教委085引导创新计划项目(085ZY1206);上海市研究生交叉创新人才培养平台项目

摘　　要：目的:探讨黄芩苷在体外对人肺癌A549细胞增殖、侵袭、迁移、对脐静脉血管内皮细胞(HUVEC)新生血管形成的影响,在体内对A549细胞裸鼠移植瘤的作用。方法:调整细胞密度为1×104/mL,应用25,50,100,200,400μmol.L-1黄芩苷作用于A549细胞24,48,72 h,采用MTT法检测黄芩苷对A549细胞的增殖抑制作用;Transwell小室法观察12.5,25,50μmol.L-1黄芩苷对A549细胞侵袭及迁移的影响;小管形成实验观察12.5,25,50μmol.L-1黄芩苷对HUVEC小管形成的影响;将A549细胞移植瘤裸鼠分为黄芩苷高剂量组(100 mg.kg-1)、黄芩苷低剂量组(50 mg.kg-1)、溶剂对照组、顺铂组(1 mg.kg-1),前3组通过ig方式给予,连续21 d;顺铂组ip连用10 d。观察黄芩苷对A549细胞裸鼠移植瘤的作用。结果:①黄芩苷能抑制A549细胞增殖,其增殖抑制率与药物浓度及作用时间呈依赖性,24,48,72 h的IC50分别为100.77,75.02,55.81μmol.L-1。②25,50μmol.L-1黄芩苷能抑制A549细胞的侵袭及迁移,从而抑制癌细胞转移。③25,50μmol.L-1黄芩苷能抑制HUVEC小管生成,与对照组比较具有统计学意义(P<0.05)。④体内实验表明50 mg.kg-1黄芩苷对A549细胞移植瘤具有一定的抑制作用。结论:黄芩苷体外可以抑制A549细胞的增殖、侵袭、迁移,抑制HUVEC小管形成,体内对A549细胞裸鼠移植瘤具有一定的抑制作用。Objective： To study the effects of baicalin on the proliferation,invasion and migration of A549 cells,and tube formation of Human umbilical vein endothelial cells（HUVEC） on matrigel in vitro,as well as the antitumor effect of baicalin on A549 cell xenografts in vivo.Method： A549 cells were treated by baicalin within different concentrations（25,50,100,200,400 μmol.L-1） 24,48,72 h respectively,the cell density was 1 × 104/ mL,cell proliferation was validated by Thiazolyl blue terazolium bromide（MTT） method.The changes of cell invasion and migration in vitro were detected by Transwell assay,the anti-angiogenic effect was detected by tube formation experiment,the concentration of baicalin of the three experiments were 12.5,25,50 μmol.L-1.Antitumor efficacy of baicalin was evaluated in nude mice models of human lung cancer xenograft,the nude mice were divided into 4 groups,the high dose group of baicalin（100 mg.kg-1）,the low dose group of baicalin（50 mg.kg-1）,the solvent control group and the cisplatin（1 mg.kg-1） group.The administration method of the three former groups was by gavage for 21 days consecutively,the cisplatin group was by intraperitoneal injection for 10 days consecutively.Result： ①After treated with different concentrations of baicalin for 24,48,72 hour,the growth of A549 cells were significantly inhibited（P〈0.05 to P〈0.01）,the IC50of baicalin in different times were 100.77,75.02,55.81 μmol.L-1 respectively.② Baicalin（25,50 μmol.L-1） could inhibit the invasion and migration of A549 cells,therefore,it inhibit the metastatic of cancer cells.③The tubule structure formation treated with baicalin（25,50 μmol.L-1） was reduced compared with the control group.④ Baicalin（50 mg.kg-1） could inhibit the A549 cell xenograft in vivo.Conclusion： These results suggest that baicalin could inhibit the proliferation,invasion and migration of A549 cells and the tube formation of Human umbilical vein endothelial cells in vitro,in vivo baicalin could inhibit t

关 键 词：黄芩苷 A549细胞 增殖 侵袭 迁移 血管生成 移植瘤 

分 类 号：R285.5[医药卫生—中药学]