狗肝菜不同相对分子质量多糖对D-GlaN致大鼠急性肝损伤的保护作用  

Protective Effects of Different Molecule Weight Range of Dicliptera chinensis Polysaccharide against D-GlaN-induced Acute Liver Injury in Rats

作　　者：赵沁元[1] 崔红花[1] 吴洁莹[1] 尹永芹[1] 沈志滨[1] 

机构地区：[1]广东药学院中药学院,广州510006

出　　处：《中国实验方剂学杂志》2013年第13期261-264,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：科技部"十二五"重大新药创制(2011ZX09102-007-03);广东省科技计划项目(2010B030700044;2011A030100013);广州市科技计划项目(12A062131631);2010年广东省教育厅高层次人才项目

摘　　要：目的:探讨狗肝菜不同相对分子质量多糖对大鼠D-半乳糖胺(D-GlaN)致急性肝损伤的保护作用。方法:采用透析的方法将狗肝菜多糖分为<6 kD,>6 kD<10 kD(6 kD～10 kD),>10 kD,以及总多糖组。D-GlaN(500 mg.kg-1)ip诱导大鼠急性肝损伤模型,动物处死后测定血清中天冬氨酸转氨酶(AST),丙氨酸转氨酶(ALT)活性,制备肝组织匀浆测定其中丙二醛(MDA)、谷胱甘肽过氧化酶(GSH-Px)含量,取肝、脾脏称重,计算脏器指数,对数据进行统计分析,并观察大鼠肝脏组织形态学变化。结果:与模型组比较,狗肝菜总多糖和相对分子质量<6 kD给药组能显著降低大鼠血清中AST,ALT活性(P<0.01);各给药组均能降低肝匀浆MDA含量(P<0.01或P<0.05),均能有效升高肝组织匀浆中GSH-Px活性(P<0.01或P<0.05),降低肝脏指数(P<0.01或P<0.05),减轻肝脏病理损伤。结论:狗肝菜各多糖给药组均有一定保肝作用,且以相对分子质量<6 kD效果最佳。Objective： This study evaluated the hepatoprotective activity of different molecule weight range of Dicliptera chinensis polysaccharide（DCP） against D-galactosamine（D-GlaN）-induced hepatotoxicity in SD rats.Method： DCP was divided into three parts according to the semipermeable membrane with different molecular weight cutoff（MWCO） using dialysis.Mw 6 kD,Mw 6 kD-10 kD,Mw 10 kD and DCP were studied on D-GlaN-induced（500 mg.kg-1 intraperitoneally） hepatic damage in rats.Liver coefficient,serum enzyms as alanine aminotransferase（ALT）,aspartate aminotransferase（AST）,hepatic tissue index malondialdehyde（MDA） and glutathione peroxidase（GSH-Px）,and histopathological Changes in liver architecture including necrosis and swelling of hepatic cells were detected.Result： Compared with the model group,pretreated with DCP and Mw 6 kD polysaccharide significantly prevented the increases of alanine ALT and AST level in serum,each group can prevented MDA accumulation,GSH-Px depletion in liver tissue,and hepatocyte swelling and necrosis.Conclusion： The results indicate that DCP can protect against D-GlaN-induced acute liver injury in rats,and the most active part is the range of molecular weight left than 6 kD.

关 键 词：狗肝菜多糖 D-半乳糖胺 急性肝损伤 

分 类 号：R285.5[医药卫生—中药学]