排钱草总生物碱对人肝星状细胞增殖及肝纤维化相关胶原蛋白、细胞因子的影响  

Effects of Total Alkaloids from Phyllodium pulchellum on Proliferation of Human Hepatic Stellate Cells and Collagen,Cytokines Related to Hepatic Fibrosis

作　　者：黄洁玲[1] 钟鸣 余胜民 

机构地区：[1]广西中医药大学,南宁530001 [2]广西壮族自治区民族医药研究院,南宁530001

出　　处：《中国实验方剂学杂志》2013年第13期283-286,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81160559)

摘　　要：目的:观察排钱草总生物碱对人肝星状细胞系Lx-2增殖及分泌肝纤维化相关胶原、细胞因子的影响。方法:Lx-2细胞调整细胞密度为1.0×105个/mL,以0.1 mL/孔接种于37℃培养,设空白对照组,排钱草总生物碱100,75,55,40,30,20,15,10 mg.L-1组,给药48 h后,采用噻唑蓝(MTT)法检测Lx-2细胞增殖;Lx-2细胞调整细胞密度为1×105个/mL,以0.1mL/孔接种于37℃培养,设空白对照及秋水仙碱(6.25 mg.L-1),排钱草总生物碱40,20,10 mg.L-1组,给药48 h后,采用酶联免疫吸附法(ELISA)法检测Lx-2细胞上清液Ⅰ型胶原(COLⅠ)、Ⅲ型胶原(COLⅢ)、Ⅳ型胶原(COLⅣ)和转化生长因子β1(TGFβ1)、血小板衍生生长因子(PDGF)含量。结果:与对照比较,排钱草总生物碱在质量浓度为100,75,55,40,30,20,15,10 mg.L-1能够浓度依赖性的抑制Lx-2细胞增殖,抑制率分别为49.4%,25.9%,24.7%,15.3%,15.3%,12.9%,2.35%,1.18%;与对照相比,排钱草总生物碱40 mg.L-1能显著抑制COLⅠ,COLⅢ,COLⅣ和TGFβ1,PDGF的分泌(P<0.05或者P<0.01)。结论:排钱草总生物碱对Lx-2细胞的增殖和COLⅠ,COLⅢ,COLⅣ,TGFβ1,PDGF的分泌有抑制作用。Objective： To observe the effect of total alkaloids from Phyllodium pulchellum on proliferation of human hepatic stellate cell line Lx-2 and the impact on secretion of collagen and cytokines which are related to liver fibrosis.Method： After recovery,Lx-2 cells in logarithmic growth were diluted to a concentration of 1 × 105/ mL,and inoculated 0.1 mL in hole in incubator at 37 ℃,blank control group was set up,the concentration of the drug administered in an order of 100,75,55,40,30,20,15,10 mg.L-1.After 48 h of administration,MTT assay was used to detect cell proliferation.Similarly,after recovery,Lx-2 cells in logarithmic growth were diluted to a concentration of 1 × 105/ mL,and inoculated 0.1 mL / hole in incubator at 37 ℃,blank control group and Colchicine group（6.25 mg.L-1） were set up.After 48 h of administration,ELISA method was used to detect Lx-2 cell supernatant for type I collagen（COL I）,type III collagen（COL III）,type IV collagen（COL Ⅳ）,transforming growth factor β1（TGF β1） and platelet-derived growth factor（PDGF） content.Result： Compared with the control group,Lx-2 cell proliferation was inhibited by the total alkaloids in a concentration-dependent manner with inhibition rates of 49.4%,25.9%,24.7%,15.3%,15.3%,12.9%,2.35% and 1.18% accordingly.Compared with the control group,the total alkaloids of 40 mg.L-1 could significantly inhibit COL I,COL Ⅲ,COL Ⅳ,TGF-β1 and PDGF secretion（P〈0.05 or P〈0.01）.Conclusion： The total alkaloids from P.pulchellum can inhibit the proliferation of Lx-2 cells and the secretion of COL Ⅰ,COL III,COL IV,TGFβ1and PDGF.

关 键 词：LX-2 排钱草总生物碱 增殖 胶原 细胞因子 

分 类 号：R285.5[医药卫生—中药学]