冷暴露大鼠下丘脑、垂体、脾淋巴细胞内神经白细胞素mRNA的变化  

Changes of neuroleukin mRNA levels in hypolhalamus, pituitary and splenic lymphocytes of rats during cold acclimation

作　　者：徐扬[1] 杨泽田 苏成芝[1] 

机构地区：[1]第四军医大学分子生物学实验室

出　　处：《第四军医大学学报》1991年第6期401-404,共4页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金 No. 3860828

摘　　要：神经白细胞素(NLK)是既作用于神经系统又作用于免疫系统的蛋白质(Mr 56 000),其表达调控于mRNA水平.150只SD纯系雄性大鼠分为对照组和冷暴露1,2,3,4周组.RNA打点杂交结果表明,大鼠冷暴露期间神经内分泌系统(垂体、下丘脑)和免疫系统(脾淋巴细胞)中 NLK mRNA的变化均一致,即RNA杂交点灰度比值:1周时增加到原值的1.63-11.31倍,2周时降低到原值的0.63倍,3周时再次增加到原值的1.92-21.09倍.这是对寒冷环境的一种普遍性反应.体外培养的淋巴细胞内合成蛋白质与上清中分泌蛋白质经SDS-PAGE放射自显影图谱分析表明淋巴细胞内NLK与其mRNA含量变化不呈平行关系,与对照组相比,冷暴露1周组NLK电泳条带极浅,而冷暴露2,3,4周组NLK放射自显影电泳条带明显增浓.这很可能是冷适应动物Ig合成增加的一个主要原因.Neuroleukin (NLK )is a protein (Mr 56 000) which acts on both the nervous and the immune systems and whose expression is regulated at mRNA levels . 150 SD male rats were divided into five groups : control group ( N ) and groups of cold - exposed 1, 2, 3, 4 weeks (C1W , C2W , C3W , C4W ). With RNA dot blot of experiments changes of NLK mRNA levels in both neuroendocrine system (pituitary and hypothalamus )and immune system ( splenic LC ) of SD male rats during cold acclimation showed similar responses , C/N ratios of scan gray intensity fevers in RNA dots as Mows : rise C/X ratio is 1.63- 11.31 in CIW, reduce C/N ratio 0.63 in C2W ana rise again C/N ratio 1.92-21.09 in C3W. It thus can be regarded as a general response to cold surroundings . SDS - PAGE autoradiogram of synthetic proteins in LC and secretory proteins in LC culture supernatant in vitro showed in a previous paper that NLK content in LC was not parallel to its mRNA levels because NLK was meagre at cold - exposed 1 week whereas it increased obviously at cold - exposed 3 and 4 weeks . This is probably a major cause of why Ig synthesis in cold - acclimated animals was greatly enhanced .

关 键 词：冷暴露 下丘脑 垂体 淋巴细胞 MRNA 

分 类 号：R339.55[医药卫生—人体生理学]