脂多糖诱发的小鼠急性肺损伤肺组织中microRNA表达谱的变化  

Expression pattern changes of microRNA in the lung tissue of mice with acute lung injury induced by lipopo- iysacclm_ride

作　　者：王斌[1] 卿娣[1] 程东良[1] 陶少华[1] 沈蔚[1] 陈衍晨[1] 杜江[1] 

机构地区：[1]南方医科大学珠江医院儿科,广州510282

出　　处：《中华实用儿科临床杂志》2013年第12期931-935,共5页Chinese Journal of Applied Clinical Pediatrics

摘　　要：目的研究脂多糖（LPS）诱导的小鼠急性肺损伤（ALI）／急性呼吸窘迫综合征（ARDS）肺组织中microRNA表达谱的改变，探讨microRNA在ALI／ARDS发生机制中的作用。方法C57BL小鼠24只，随机分为实验组和对照组，每组12只。实验组小鼠通过气管切开经呼吸道向肺内注入LPS（6mg／kg），构建小鼠ALI/ARDS模型。对照组用同样的方法注入相应剂量的9g／L盐水。所有小鼠均于手术后24h处死，取左肺组织用于测量干湿质量比，右中肺叶行HE染色观察病理变化。应用microRNA芯片技术检测对照组及实验组小鼠肺组织microRNA表达谱的差异，用生物信息学方法进行靶基因预测及信号通路分析。结果与对照组比较，实验组小鼠出现明显的呼吸系统症状，肺组织干湿质量比明显升高（P〈0．01），病理结果符合ALI／ARDS特征。芯片结果显示ALI／ARDS小鼠肺组织中明显差异表达的microRNA有48个，其中上调的有27个，下调的有21个，这些microRNA的靶基因可能参与调节炎症相关的信号通路。结论在ALI／ARDS小鼠模型中，部分microRNA的表达发生明显的变化，microRNA在ALI／ARDS病理生理过程中起重要作用。Objective To investigate the change of expression pattern of microRNA in the lung with acute lung injury （ALI）/acute respiratory distress syndrome（ARDS） induced by lipopolysaccharide （LPS）, and to provide an evidence that mieroRNAs are involved in the pathogenesis of ALI/ARDS. Methods Twenty-four C57BL mice were randomly divided into control group and LPS treated group, 12 mice in each group. The rats in LPS treated group were treated with intratracheal injection of LPS at a dose of 10 mg/kg body weight into the lung. The rats in control group were treated with the same dose of saline instead. All mice were sacrificed 24 hours after operation, the left lung was ex- cised to measure the wet-to-dry weight （W/D） ratio,and the right upper lobe was stained with hematoxylin and eosin （HE）. A mieroRNA microarray chip was used to profile miRNA expressions in the lung of rats in both LPS treated group and control group. Online software packages were used to predict the gene targeted by microRNAs. Results Compared with the control group, the LPS treated mice had obvious respiratory symptoms, the W/D ratio was significant- ly increased （P 〈 0.01 ） ,and the pathology was characterized with ALI/ARDS. The microarray chip results demonstra- ted that the expressions of 48 mieroRNAs were significantly changed in the ALI/ARDS mice. Among these miRNA,27 cases were up-regulated ,21 eases were down-regulated. The target genes of these mieroRNAs might be involved in regu- lating the signal pathway of inflammation. Conclusions Some miRNAs express differently in the model of ALI/ARDS, and they may play an important role in pathophysiologieal process of ALI/ARDS.

关 键 词：MICRORNA 急性肺损伤 急性呼吸窘迫综合征 

分 类 号：R563.8[医药卫生—呼吸系统]