转小萝卜γ-硫堇蛋白基因RsAFP1水稻的获得及其稻瘟病抗性初步鉴定  

Transformation of γ-thionin Gene RsAFP1 into Rice and Preliminary Idenfication of Its Blast Resistance

作　　者：李海青[1,2] 李臻[1] 王莹莹[1] 柳絮[1] 王庆国[1] 姚方印[1] 刘炜[1,2] 

机构地区：[1]山东省农业科学院高新技术研究中心/山东省作物遗传改良与生态生理重点实验室,济南250100 [2]山东师范大学生命科学学院,济南250014

出　　处：《中国水稻科学》2013年第4期335-343,共9页Chinese Journal of Rice Science

基　　金：国家转基因植物新品种培育重大专项(2009ZX08001-010B)

摘　　要：将从小萝卜中分离的硫堇蛋白类基因RsAFP1通过基因工程的方法导入带有泛素Ubiquitin启动子及抗除草剂Bar基因的植物双元表达载体pCAMBIA1300中,经农杆菌介导对圣稻13进行遗传转化。对获得的阳性转基因植株进行稻瘟病菌体外及室内苗期稻瘟病菌接种抗性鉴定,结合田间自然发病情况,对转基因植株的抗稻瘟病性进行综合评价。初步结果表明,外源RsAFP1基因的导入可在一定程度上提高水稻对稻瘟病的抗性。A γ-thionin protein gene termed RsAFP1 isolated from the radish by PCR was introduced into the modified binary vector pCAMBIA1300.Six positive transformants were obtained by agrobacterium-mediated transformation to rice callus.Further,the in vitro bacterial inhibition test and indoor rice blast fungus inoculation to rice seedling were conducted.In combination with the natural incidence in field,the resistance of transgenic lines were comprehensive evaluated.The results showed that introduction of RsAFP1 gene could improve rice blast resistance.

关 键 词：稻瘟病 γ-硫堇蛋白 RsAFP1基因 抗病性 

分 类 号：S511[农业科学—作物学]