NLRP3炎症小体及其下游分子在幽门螺杆菌感染小鼠的表达  

The expressions of NLRP3 inflammasome and its downstream molecules in the mouse model of Helicobacter pylori infection

作　　者：蒋建烨[1] 刘胜[1] 罗晶晶[1] 李翔[1] 唐双阳[1] 余敏君[1] 蔡恒玲[1] 田湉[1] 张艳[1] 

机构地区：[1]南华大学病原生物学研究所

出　　处：《细胞与分子免疫学杂志》2013年第8期785-788,793,共5页Chinese Journal of Cellular and Molecular Immunology

基　　金：湖南省教育厅重点项目(11A103);湖南省大学生研究性学习和创新性实验计划项目(湘教通[2011]272号)

摘　　要：目的研究NLRP3炎症小体信号通路相关分子在幽门螺杆菌(H.pylori)感染C57BL/6小鼠胃组织和血清中的表达情况,初步探讨NLRP3炎症小体信号通路在H.pylori致病中的作用。方法将C57BL/6小鼠分为H.pylori感染组和PBS对照组,于感染后不同时间点处死小鼠,RT-PCR检测小鼠胃组织中NLRP3炎症小体和下游信号通路分子mRNA表达,Western blot法检测小鼠胃组织中caspase-1蛋白表达,ELISA测定小鼠血清中IL-1β、IL-18和IL-33含量。结果与对照组相比,H.pylori感染组小鼠胃黏膜均呈慢性炎症改变,且随时间延长,炎症程度逐渐加重。NLRP3炎症小体信号通路相关分子mRNA及caspase-1蛋白表达水平显著升高,血清中IL-1β、IL-18、IL-33的含量均显著增高。结论 H.pyori感染可以激活NLRP3炎症小体信号通路。Objective To study the expressions of NLRP3 inflammasome and its downstream molecules in the gastric tis-sues and sera of Helicobacter pylori （ H. pylori）-infected C57BL/6 mice, and to preliminary explore the role of NLRP3 inflam-masome signalling pathway in the pathogenesis of H. pylori infection. Methods C57BL/6 mice were randomly divided into H. pylori infection group and PBS control group, and mice were sacrificed at different time points after H. py/ori infection. The mRNA expressions of NLRP3 inflammasome and downstream signalling pathway related molecules in mouse gastric tissues were detected by RT-PCR, and the protein level of caspase-1 was analyzed by Western blotting. The contents of IL-1β, IL-18 and IL-33 in the sera were measured with ELISA. Results Chronic inflammatory response was observed in the gastric mucosal tissues of H. pylori-infected mice and it gradually aggravated. Compared with the control mice, the mRNA expres-sions of NLRP3 inflammasome signalling pathway related molecules and the protein level of caspase-1 increased markedly in the gastric tissues of H. pylori-infected mice. Moreover, the contents of IL-1β, IL-18 and IL-33 in the sera of H. pylori-infected mice were also significantly elevated. Conclusion NLRP3 inflammasome signalling pathway could be activated by H. pylori infection.

关 键 词：幽门螺杆菌 NLRP3 炎症小体 IL-1Β IL-18 IL-33 

分 类 号：R392-33[医药卫生—免疫学] R392.1[医药卫生—基础医学]