Rab23在人乳腺癌细胞Bcap-37侵袭迁移中的作用  

Role of Rab23 in invasion and migration of human breast cancer Bcap-37 cells

作　　者：赵洁[1] 刘亚莉[1] 简强[2] 李娟[1] 迟素敏[1] 

机构地区：[1]第四军医大学基础部生理学教研室 [2]第四军医大学西京医院皮肤科,陕西西安710032

出　　处：《细胞与分子免疫学杂志》2013年第8期813-817,共5页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(31200876;81272984;30672368)

摘　　要：目的探讨过表达和敲除Rab23对人乳腺癌Bcap-37细胞侵袭迁移能力的影响。方法应用Western blot法检测乳腺细胞HBL-100及乳腺癌细胞Bcap-37、MDA-MB-231、MCF-7中Rab23的表达情况。慢病毒转染乳腺癌细胞Bcap-37,筛选出稳定过表达和敲除Rab23的Bcap-37细胞。划痕愈合试验、Transwell实验检测过表达和敲除Rab23后Bcap-37细胞侵袭迁移能力的变化。结果 Western blot法结果显示,Rab23在乳腺和乳腺癌细胞系中均有表达,且Bcap-37细胞表达水平最高,与HBL-100细胞相比,差异有统计学意义(P<0.01);与空载体Bcap-37细胞比较,过表达Rab23的Bcap-37细胞侵袭迁移能力明显增强(P<0.01);敲除Rab23的Bcap-37细胞侵袭迁移能力明显减弱(P<0.01)。结论 Rab23在乳腺癌细胞Bcap-37的侵袭迁移中发挥着重要作用。Objective To investigate the effects of Rab23 over-expression and Rab23 knock-out on the migration and invasion of breast cancer cell line Bcap-37. Methods Western blotting was employed to detect the expression of Rab23 in breast cell line HBL-100 and cancer cell lines Bcap-37, MDA-MB-231, and MCF-7. Lentiviral vectors with Rab23 genes and with Rab23 RNAi were respectively transfected into breast cancer cell line Bcap-37, and the cells presenting the stable over-expression of Rab23 and the knock-out of Rab23 were respectively selected. Scratch healing assay and Transwell invasion assay were used to examine the alterations of Bcap-37 cell migration and invasion potentials after Rab23 over-expression and Rab23 knock-out. Results Western blotting indicated that Rab2.3 was expressed in all breast cancer cell lines and normal breast cell line. And Rab23 expression level was the highest in Bcap-37 cells, which was statistically different as compared with HBL-100 cells （ P 〈 0.01）. Compared with the control group, Bcap-37 cell line with Rab23 over-expression had the dramatically enhanced migration and invasion potentials （P 〈 0.01）. On the other hand, Bcap-37 cell line with Rab23 knock-out showed the significantly attenuated migration and invasion potentials （P 〈0. 01 ）. Conclusion Rab23 plays a pivotal role in the migration and invasion of Bcap-37 cells.

关 键 词：Rab23 慢病毒转染 乳腺癌 侵袭 迁移 

分 类 号：R392-33[医药卫生—免疫学] R737.9[医药卫生—基础医学]