肺结核患者痰液细胞和血清中miR-618的水平变化及功能预测  

Expression and function prediction of miR-618 in sputa and sera of patients with pulmonary tuberculosis

作　　者：伊正君[1] 付玉荣[1] 吴洪娟[1] 赵山[1] 

机构地区：[1]潍坊医学院临床检验诊断学重点实验室,附属医院检验科,山东潍坊261031

出　　处：《细胞与分子免疫学杂志》2013年第8期846-849,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(81100006);山东省自然科学基金(ZR2010HM073)

摘　　要：目的检测肺结核患者痰液和血清中miR-618的水平变化及miR-618靶基因细胞因子IL-13的含量,并用生物信息学技术和软件对miR-618的功能进行预测分析。方法收集健康对照和活动性肺结核患者的痰液和血清,用核酸杂交和实时定量PCR(qRT-PCR)检测痰液和血清中miR-618的水平。用ELISA检测IL-13的含量。TargetScan预测miR-618的靶基因,用DAVID数据库和Cytoscape的BINGO插件对miR-618的预测靶基因进行GO注释分析和生物通路分析。结果杂交和qRT-PCR结果显示:miR-618在活动性肺结核患者痰液和血清中的水平均比健康对照组显著降低(P<0.05)。IL-13含量虽然在对照组和结核组中无统计学差异,但在结核组有增高的趋势。miR-618预测靶基因的GO注释结果富集在转录调控等生物学过程、DNA结合等分子功能等方面(P<0.05);在KEGG通路数据库中miR-618a预测靶基因主要富集于TGF-β信号通路中(P<0.05)。结论 miR-618在活动性肺结核感染患者痰液和血清中的含量都明显降低,miR-618的靶基因主要参与转录调控、RNA代谢等生物学过程。Objective To measure the level of miR-618 in sputa and sere from the patients with pulmonary tuberculosis, determine the concentration of IL-13 which is a cytokine encoded by target gene of miR-618, and predict the function of miR-618 with bioinformatics. Methods Sputum and serum samples were collected from the healthy controls and the patients with active pulmonary tuberculosis, respectively. Level of miR-618 was measured by nucleic acid hybridization and real-time quan-titative RT-PCR（qRT-PCR）. Concentration of IL-13 was determined by ELISA. Target genes of miR-618 were predicted with TargetSean, and GO annotation and biological pathway of the target genes were analyzed with DAVID database and BINGO of Cytoscape. Results Both hybridization and qRT-PCR results showed that level of miR-618 in both sputa and sera obviously decreased in the tuberculosis group compared to the control group （ P 〈 0.05）. There was no statistical difference in IL-13 concentration between the tuberculosis group and the control group, however, it had an increasing tendency in the former. GO annotation of predicted target genes of miR-618 was enriched in biological processes such as regulation of transcription and molecular functions such as DNA binding （ P 〈 0.05）. In KEGG pathway, the predicted target genes mostly existed in TGF-β signaling pathway （ P 〈 0. 05）. Condudon Level of miR-618 in both sputa and sera was significantly lower in the tuberculosis group than that in the control group and predicted target genes of miR-618 were mainly involved in biological processes such as regulation of transcription and RNA metabolism.

关 键 词：肺结核 miR-618 IL-13 靶基因 生物信息学 

分 类 号：R392.11[医药卫生—免疫学] R378[医药卫生—基础医学]