空间毛细管式蛋白质结晶室样品加载技术研究  

Study on Loading Techniques of Protein Species into Space Crystallization Chamber

作　　者：李娜[1] 张贺桥[1] 尚桂军[1] 聂荣鑫[1] 马建华[1] 仓怀兴[1] 

机构地区：[1]中国科学院生物物理研究所,北京100101

出　　处：《空间科学学报》2013年第4期441-447,共7页Chinese Journal of Space Science

基　　金：载人航天工程应用系统项目资助

摘　　要：采用X-射线衍射技术研究蛋白质分子结构与功能的必要前提是获得高质量的蛋白质晶体.空间微重力环境是生长优质蛋白质晶体的理想场所.蛋白质样品的加载工艺对于空间蛋白质结晶实验的成效具有重要影响.针对为神舟八号飞船空间实验新研制的毛细管式空间蛋白质结晶室,结合样品加载基本流程,对加载工艺和伴随的气泡缺陷问题进行了系统和深入分析,确定了针头形状、毛细管封口质量和硅化效果、样品加载工具以及毛细管夹持方式等影响因素,并获得了实验测试验证.在此基础上,通过改进毛细管烧制工艺和样品加载工具,研制和使用专用毛细管夹具等措施,简化了蛋白质样品加载工艺,消除了气泡缺陷,提高了加载效率.新工艺的实施保证了空间实验任务的顺利完成.The prerequisite of using X-ray diffracting technique to study the relation between protein molecular structure and its function is to obtain the protein crystals of high quality. The microgravity environment of space is an ideal place for culturing such protein crystals. The experimental yield of the space protein crystallization is affected heavily by the loading techniques of the protein species into the crystallization chamber. The loading technique of protein species into the new-designed crystallization chamber for protein crystallization experiment aboard Shenzhou-8 spaceship is studied systematically. It is deduced that the needle shape of the pipette, the protein loading tool, the holding manner of the glass capillary tube, the qualities of sealing and siliconization of the tube have remarkable influences on the experimental results of protein crystallization, which were proved to result in the inclusion of gas bubbles. The corresponding measures such as improving sealing technique and protein loading pipette, constructing a tube-holding tool, are adopted to optimize loading technique and eliminate the gas bubbles. The new techniques have been implemented successfully to the final space experiment of protein crystallization aboard Shenzhou-8 spaceship.

关 键 词：空间蛋白质结晶 结晶室 加载工艺 微重力 气泡 

分 类 号：Q51-3[生物学—生物化学] Q693