Digestive stability and acute toxicity studies of exogenous protein in transgenic rice expressing lysine-rich fusion proteins  

作　　者：ZHAO XiangXiang HU XiaoLan TANG Tang LU ChangLi LIU FuXia JI LiLian LIU QiaoQuan 

机构地区：[1]Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, Huaiyin Normal University [2]Key Laboratory of Crop Genetics and Physiology of the Jiangsu Province and Plant Functional Genomics of the Ministry of Education, Yangzhou University

出　　处：《Chinese Science Bulletin》2013年第20期2460-2468,共9页

基　　金：supported by grants from the National Major Special Project for the Development of Transgenic Organisms (2009ZX-08011-003B);Jiangsu Province Qing Lan Project for Young and Middle-aged academic leaders;Jiangsu Province Qing Lan Project for Outstanding Scientific and Technological Innovation Team

摘　　要：Evaluating exogenous protein expressed in transgenic crops is one of the most effective methods of assessing the safety of transgenic plants. The objective of this study was to assess the food safety of genetically modified (GM) rice containing a lysine-rich fusion protein gene (transgenic GL gene rice) by in vitro digestion and acute toxicity testing of exogenous protein, according to the national standard of the People's Republic of China. The exogenous protein was rapidly degraded in the simulated gastric and intestinal fluids. In the acute experiment, the exogenous protein was injected into Institute of Cancer Research (ICR) mice via the tail vein at a dose of 438 mg kg-1 body weight. No adverse effects on animal behavior or mortality were observed during the following 15-day period and there were no significant biological changes in body weight, serum biochemistry parameters, relative organ weights or histopathological examinations, compared with the control group. Therefore, exogenous protein in transgenic GL gene rice has a low potential allergenicity or toxicity risk.Evaluating exogenous protein expressed in transgenic crops is one of the most effective methods of assessing the safety of transgenic plants. The objective of this study was to assess the food safety of genetically modified （GM） rice containing a lysine-rich fusion protein gene （transgenic GL gene rice） by in vitro digestion and acute toxicity testing of exogenous protein, according to the national standard of the People＇ s Republic of China. The exogenous protein was rapidly degraded in the simulated gastric and intestinal fluids. In the acute experiment, the exogenous protein was injected into Institute of Cancer Research （ICR） mice via the tail vein at a dose of 438 mg kg-1 body weight. No adverse effects on animal behavior or mortality were observed during the following 15-day period and there were no significant biological changes in body weight, serum biochemistry parameters, relative organ weights or histopathological examinations, compared with the control group. Therefore, exogenous protein in transgenic GL gene rice has a low potential allergenicity or toxicity risk.

关 键 词：急性毒性试验 融合蛋白基因 转基因水稻 外源蛋白质 体外消化 赖氨酸 稳定性 组织病理学检查 

分 类 号：S511[农业科学—作物学]