Star-shaped conjugated oligoelectrolyte for bioimaging in living cells  

作　　者：SONG WenLi JIANG RongCui YUAN Yan LU XiaoMei HU WenBo FAN QuLi HUANG Wei 

机构地区：[1]Key Laboratory for Organic Electronics & Information Displays (KLOEID) and Institute of Advanced Materials (IAM), Nanjing University of Posts & Telecommunications (NJUPT)

出　　处：《Chinese Science Bulletin》2013年第21期2570-2575,共6页

基　　金：supported by the National Basic Research Program of China (2009CB930600, 2012CB933301 and 2012CB723402);the Ministry of Education of China (IRT1148);the National Natural Science Foundation of China (21222404, 51173080 and 21104033);the Program for New Century Excellent Talents in University (NCET-10-0179);the Specialized Research Fund for the Doctoral Program of Higher Education(20093223110003);the Natural Science Foundation of Jiangsu Province of China (BZ2010043 and NY211003);the Program for Postgraduates Research Innovation of Colleges;Universities of Jiangsu Province(CXLX11_0424 and CXLX11_ 0424_0414)

摘　　要：A new star-shaped oligoelectrolyte (TEFCOONa) with triphenylamine as the core, acetylene as linkage and anionic fluorenes as arms was obtained and used for direct imaging in living PANC-1 cells. Because of the hydrophobic conjugated groups of the oli-goelectrolyte, TEFCOONa can form nanospheres with an average diameter of^75 nm in 10 mmol/L PBS. These nanospheres possess a relatively high absolute quantum yield (16.5% in PBS), low cytotoxicity and can penetrate into the nucleus through the cytoplasm, which is essential for living cellular imaging. Collectively, these results validate our rational design of conjugated oligoelectrolyte and even hyper branched polymers-copolyelectrolyte as effective nanovectors for bioimaging and other clinical applications.A new star-shaped oligoelectrolyte （TEFCOONa） with triphenylamine as the core, acetylene as linkage and anionic fluorenes as arms was obtained and used for direct imaging in living PANC-1 cells. Because of the hydrophobic conjugated groups of the oli- goelectrolyte, TEFCOONa can form nanospheres with an average diameter of -75 nm in 10 mmol/L PBS. These nanospheres possess a relatively high absolute quantum yield （16.5% in PBS）, low cytotoxicity and can penetrate into the nucleus through the cytoplasm, which is essential for living cellular imaging. Collectively, these results validate our rational design of conjugated oligoelectrolyte and even hyper branched polymers-copolyelectrolyte as effective nanovectors for bioimaging and other clinical applications.

关 键 词：活细胞成像 生物成像 共轭 星形 超支化聚合物 直接成像 平均直径 纳米微球 

分 类 号：TQ460.1[化学工程—制药化工]