Programmed self-assembly of DNA origami nanoblocks into anisotropic higher-order nanopatterns  

作　　者：FU YanMing CHAO Jie LIU HuaJie FAN ChunHai 

机构地区：[1]Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences

出　　处：《Chinese Science Bulletin》2013年第21期2646-2650,共5页

基　　金：supported by the National Basic Research Program of China (2012CB932600);the National Natural Science Foundation of China(20725516, 90913014, 21028005 and 21103219);Shanghai Pujiang Program (11PJ1412000)

摘　　要：Anisotropic nanopatterns have potentials in constructing novel plasmonic structures which have various applications in such as super-resolution microscopy, medicine, and sensors. However, it remains challenging to build big anisotropic nanopatterns that are suitable for big noble metal nanoparticles. Herein, we report a simple and reliable strategy for constructing DNA origami-based big anisotropic nanopatterns with controlled size and shape, nanoscale resolution, and fully addressability. Two kinds of basic DNA origami nanoblocks-cross-shaped and rectangular DNA origami units were used. We have demonstrated that by encoding nanoblocks' edges, anisotropic higher-order nanopatterns, such as dimer, trimer, tetramer and mini "windmill" like pentamer nanopatterns could be constructed. To show the potential use as template to direct the assembly of anisotropic nanoparticles arrays, a proof of concept work was conducted by anchoring streptavidin nanoparticles on the "windmill" template to form a chiral array. Significantly, these nanopatterns have the sizes of hundreds of nanometers, which are in principle also suitable for big noble metal nanoparticles arrays.Anisotropic nanopattems have potentials in constructing novel plasmonic structures which have various applications in such as super-resolution microscopy, medicine, and sensors. However, it remains challenging to build big anisotropic nanopattems that are suitable for big noble metal nanoparticles. Herein, we report a simple and reliable strategy for constructing DNA origa- mi-based big anisotropic nanopatterns with controlled size and shape, nanoscale resolution, and fully addressability. Two kinds of basic DNA origami nanoblocks - cross-shaped and rectangular DNA origami units were used. We have demonstrated that by encoding nanoblocks＇ edges, anisotropic higher-order nanopatterns, such as dimer, trimer, tetramer and mini ＂windmill＂ like pen- tamer nanopatterns could be constructed. To show the potential use as template to direct the assembly of anisotropic nanoparticles arrays, a proof of concept work was conducted by anchoring streptavidin nanoparticles on the ＂windmill＂ template to form a chi- ral array. Significantly, these nanopatterns have the sizes of hundreds of nanometers, which are in principle also suitable for big noble metal nanoparticles arrays.

关 键 词：大各向异性 体结构 DNA 折纸 高阶 金属纳米粒子 自组装 程序化 

分 类 号：Q819[生物学—生物工程]