Generalized multiparticle Mie modeling of light scattering by cells  

作　　者：WANG Meng CAO Min GUO ZhiRui GU Ning 

机构地区：[1]State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory of Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University [2]The Second Affiliated Hospital of Nanjing Medical University

出　　处：《Chinese Science Bulletin》2013年第21期2663-2666,共4页

基　　金：supported by the National Basic Research Program of China (2011CB933500);the National Natural Science Foundation of China (60725101, 50872021);the Basic Research Program ofJiangsu Province (BK2009013)

摘　　要：The development of nanobiology requires a fundamental understanding of the interaction features between light and cells as well as cells containing nanoparticles. In this study, the generalized multiparticle Mie (GMM) theory was employed to calculate the scattering properties of cells under refractive index matching conditions. The angular distribution of scattered light is statistically averaged to obtain a good fit for the experimental results. Based on a simplified cell model, the variabilities between the scattered light pattern of normal cells and that of cancerous cells were examined. The results indicate that the small angle scattering is sensitive to the organelle distribution, which could be applied in the diagnostics of cancerous cells. Finally, the effects of cellular uptake of nanoparticles on the scattering pattern was also investigated.The development of nanobiology requires a fundamental understanding of the interaction features between light and cells as well as cells containing nanoparticles. In this study, the generalized multiparticle Mie （GMM） theory was employed to calculate the scattering properties of cells under refractive index matching conditions. The angular distribution of scattered light is statistically averaged to obtain a good fit for the experimental results. Based on a simplified cell model, the variabilities between the scattered light pattern of normal cells and that of cancerous cells were examined. The results indicate that the small angle scattering is sensitive to the organelle distribution, which could be applied in the diagnostics of cancerous cells. Finally, the effects of cellular uptake of nanoparticles on the scattering pattern was also investigated.

关 键 词：光散射 多粒子 广义 细胞模型 建模 纳米粒子 纳米生物学 折射率匹配 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学] TB383.1[医药卫生—基础医学]