不同性别小鼠肝脏中Ⅱ相代谢酶的mRNA表达  

mRNA Profiles for Phase II Metabolizing Enzymes in Livers of Male and Female Mice

作　　者：刘莹[1] 刘楠启[1] 蒋昆谕[2] 孟胜男[2] 

机构地区：[1]中国医科大学基础医学院生物化学教研室,沈阳110001 [2]中国医科大学药学院药剂学教研室,沈阳110001

出　　处：《中国细胞生物学学报》2013年第7期937-942,共6页Chinese Journal of Cell Biology

基　　金：国家自然科学基金(批准号:81173123)资助的课题~~

摘　　要：该研究考察了性别差异对小鼠肝脏Ⅱ相代谢酶的mRNA表达的影响。将6月龄不同性别的C57BL/6小鼠分为雌、雄两组(n=6),分别取其肝脏。以荧光定量PCR(Real-time quantitative PCR,RT-qPCR)测定肝脏中II相代谢酶mRNA的相对表达量。在分析的19个II相代谢酶中,有4个代谢酶(Ugt1a1、Sult1a1、Sult2a2和Sult3a1)的mRNA水平在雌性小鼠肝脏中高表达(P<0.01或P<0.05);6个代谢酶(Ugt2b1、Ugt2b5、Ugt2b35、Sult5a1、Gstp1和Gstp2)的mRNA水平在雄性小鼠肝脏中表达较高(P<0.01或P<0.05);其他检测的II相代谢酶在雌雄小鼠肝脏中的mRNA表达没有显著性差异。性别差异显著影响小鼠肝脏中II相代谢酶的mRNA表达,这将导致药物在药效和毒性方面的个体差异。Gender differences in mRNA profiles for phase Ⅱ metabolizing enzymes were characterized in livers of male and female mice of 6 months of age. C57BL/6 mice were randomly divided into female group and male group （n=6）, the livers were collected. The relative mRNA expression of phase Ⅱ metabolizing enzymes was examined by Real-time quantitative PCR （RT-qPCR）. Gender differences were investigated for 19 phase Ⅱ metabolizing enzymes. In female and male mice, mRNA levels for 4 phase Ⅱ metabolizing enzymes （e.g., Ugtlal, Sultlal, Sult2a2 and Sult3al） were higher in female mice （P〈0.01 or P〈0.05）, whereas mRNA levels for 6 phase Ⅱ metabolizing enzymes （e.g., Ugt2bl, Ugt2b5, Ugt2b35, Sult5al, Gstpl and Gstp2） were higher in male mice （P〈0.01 or P〈0.05）. The mRNA levels of the other enzymes showed similar levels in both genders. Gender differences have significant effects on the mRNA expression profiles of phase Ⅱ metabolizing enzymes in livers of mice, and it may contribute to individual differences in drug efficacy and toxicity.

关 键 词：Ⅱ相代谢酶 mRNA谱 C57BL 6小鼠 性别差异 

分 类 号：R96[医药卫生—药理学]