中药小复方(液)对麻黄素致损伤中肾SOD、CAT活性及Bax蛋白与TGF-β1表达的影响  

The Effect of Chinese Medicine (Compound) on SOD,CAT Activities and Bax Protein,TGF-β1 Expression of Filial Mice Kidney Injured by Ephedrine

作　　者：王锦锦[1] 李重阳[2] 俞诗源[1] 孙隽[1] 

机构地区：[1]西北师范大学生命科学学院,兰州730070 [2]甘肃政法学院,兰州730070

出　　处：《中国细胞生物学学报》2013年第7期943-949,共7页Chinese Journal of Cell Biology

基　　金：国家自然科学基金(批准号:30370163);甘肃省自然科学基金(批准号:1107RJZA141);兰州市社会发展项目(批准号:2010-A127)资助的课题~~

摘　　要：研究中草药对兴奋剂损伤的保护作用。麻黄素组用递增剂量连续腹腔注射0.2 mL(2.0,3.0,4.0 g/L)的麻黄素溶液,中药组在注射麻黄素后分别灌胃0.2 mL(20.0,30.0,40.0 g/L)的中药小复方(液),分别于给药5,10,15 d后用比色法检测小鼠肾组织SOD、CAT活性及MDA含量,用免疫组织化学法检测肾组织中Bax蛋白和TGF-β1的表达情况。麻黄素组肾脏中丙二醛含量升高(P<0.05或P<0.01),超氧化物歧化酶和过氧化氢酶活性降低(P<0.05或P<0.01),肾组织Bax蛋白和TGF-β1的阳性表达增强;中药组肾脏中丙二醛含量又降低(P<0.05或P<0.01),超氧化物歧化酶和过氧化氢酶活性又回升(P<0.05或P<0.01),肾组织Bax蛋白和TGF-β1的阳性表达强度降低。中药小复方(液)可提高组织细胞的抗氧化能力,抑制细胞脂质过氧化,抑制Bax蛋白和TGF-β1的表达。中药小复方(液)对于麻黄素致小鼠组织的损伤可能有一定的治疗或保护作用。To explore protection of Chinese medicine against stimulant injury, the ephedrine group intraperitoneal injected 0.2 mL escalation doses （2.0, 3.0, 4.0 g/L） of ephedrine, and Chinese medicine group intragastric with 0.2 mL the concentration of 20.0, 30.0, 40.0 g/L of Chinese medicine （compound） after injected ephedrine 1 h respectively. At 5, 10, 15 d, the activities of SOD, CAT as well as content of MDA were detected by colorimetry respectively. In addition, the expression of Bax protein and TGF-β1 were measured by immunohistochemistry in kidney of mice at the different developmental stages. Results showed the content of MDA in ephedrine group increased （P〈0.05, P〈0.01）; activity of SOD and CAT decreased （P〈0.05, P〈0.01）; the expression of Bax protein and TGF-β1 increased in kidney. The content of MDA in Chinese medicine group decreased （P〈0.05, P〈0.01）; activity of SOD and CAT increased （P〈0.05, P〈0.01）; the expression of Bax protein and TGF-β1 decreased in kidney. The Chinese medicine （compound） could improve the cell anti-oxidantion, inhibitcell lipd peroxidation and the expression of Bax and TGF-β1, promote the organization cell repair and reduce cell damage by improving cell activity and inhibiting apoptosis. The Chinese medicine （compound） helps to protect and cue ephedrine-caused tissue damage.

关 键 词：中药小复方(液) 麻黄素 小鼠 肾脏 抗氧化物酶 

分 类 号：R285.5[医药卫生—中药学]