CCR7基因rs3136685多态性与中国北方汉族人群冠心病的关系  

Relationship between CCR7 rs3136685 polymorphism and coronary artery disease in Han population in north China

作　　者：蔡文芝[1] 张效林[2] 冯雪瑶[2] 白净[2] 刘腾飞[1] 汪洁[2] 韩雅玲[2] 

机构地区：[1]辽宁医学院研究生学院,辽宁锦州121000 [2]沈阳军区总医院心内科,沈阳110840

出　　处：《解放军医学杂志》2013年第7期561-564,共4页Medical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金青年科学基金项目(81100135);军队临床高新技术重大项目(2010GXJS001)~~

摘　　要：目的探讨趋化因子CCR7基因单核苷酸多态位点rs3136685多态性与中国北方汉族人群冠心病的关系。方法连续收集2010年3月-2011年12月沈阳军区总医院心内科住院患者578例,其中病例组(经冠状动脉造影证实一支或多支血管狭窄50%以上)287例,对照组(冠状动脉造影正常且没有其他粥样硬化证据)291例。冠心病组根据血管病变支数分为3个亚组:单只病变组(n=104),双支病变组(n=89)和3支病变组(n=94)。提取患者血液白细胞基因DNA,采用直接测序法测定趋化因子CCR7基因rs3136685单核苷酸多态位点的基因型,并进行基因型和等位基因与冠心病及冠心病病变程度的相关性分析。结果 CCR7基因rs3136685单核苷酸多态性共检测到3种基因型,即GG型、AG型和AA型,在病例组的分布频率分别为42.9%、45.6%、11.5%,在对照组分别为43.0%、44.3%、12.7%,两组基因型分布皆符合Hardy-Weinberg平衡定律,3种基因型在两组间的分布差异无统计学意义(P>0.05)。A等位基因在病例组及对照组的分布频率分别为34.3%、34.9%,差异亦无统计学意义(P>0.05)。亚组分析显示,CCR7基因rs3136685单核苷酸多态性的基因型和等位基因频率在单支病变、双支病变及3支病变3个亚组间差异无统计学意义(P>0.05)。结论 CCR7基因rs3136685单核苷酸多态性与中国北方汉族人群冠心病的发病及疾病严重程度可能无相关关系。Objective To explore the relationship between the polymorphism of CCR7 rs3136685 and the coronary artery disease（CAD） in Han population in north China.Methods A total of 578 cases（including 287 patients with CAD and 291 control individuals） admitted to General Hospital of Shenyang Military Command from Mar.2010 to Dec.2011 were enrolled in the present study.All the subjects were unrelated individuals of Chinese Han inhabited in the northeast area of China,and had undergone coronary angiography.Those with 50% or more angiostenosis in one or more coronary arteries were sorted out as CAD group,which were then divided in 3 subgroups： namely angiostenosis in one vessel（n=104）,in two vessels（n=89） and in three vessels（n=94）.Those with normal angiography and no evidence of atherosclerosis were grouped as controls.Genomic DNA was extracted from blood leukocytes and polymorphic genotypes were determined by polymerase chain reaction and sequencing analysis.The correlation of rs3136685 genotype and allele frequency to CAD and the angiographic severity of CAD were analyzed.Results Three genotypes（GG,AG and AA） were detected in CCR7 rs3136685 genetic locus,their frequencies were 42.9%,45.6% and 11.5%,respectively,in CAD group;and 43.0%,44.3% and 12.7%,respectively,in control group（P＆gt;0.05）.The distribution of the 3 genotypes in the both groups was coincident with the law of equilibrium.The distribution frequencies of A allele in CAD group and control group were 34.3% and 34.9% respectively,with no significant difference（P＆gt;0.05）.Further stratification analysis among the polymorphism of CCR7 rs3136685 and angiographic severity of CAD also yielded negative results（P＆gt;0.05）.Conclusion TheCCR7 rs3136685 polymorphism is unlikely to be a major contributor to the pathogenesis of CAD in Han population in north China.

关 键 词：炎症趋化因子类 多态性 单核苷酸 冠状动脉疾病 

分 类 号：R541.4[医药卫生—心血管疾病]