亚磁生物学效应研究的磁屏蔽培养系统研制  

A Magnetic Shielded Incubation System for Investigating Biological Effects of Hypomagnetic Field

作　　者：莫炜川[1] 刘缨[1] 蒋远大[2] 翟光杰[2] 赫荣乔[1] 

机构地区：[1]中国科学院生物物理研究所脑与认知国家重点实验室,北京100101 [2]中国科学院空间科学与应用研究中心,北京100190

出　　处：《航天医学与医学工程》2013年第3期249-253,共5页Space Medicine & Medical Engineering

基　　金：中国科学院科研装备研制项目(YZ201148);国家自然科学基金青年科学基金项目(31200628);中国科学院与昆士兰州生物技术合作计划(GJHZ1131)

摘　　要：目的在地面模拟空间亚磁环境,为亚磁场的生物学效应及其防护措施的研究提供技术平台。方法将坡镆合金磁屏蔽箱放入细胞培养箱中,平衡磁屏蔽箱以及细胞培养箱中的培养条件,形成亚磁培养环境。结果该磁屏蔽培养系统能够提供0～600 nT范围的亚磁场,以及9～60μT范围的多梯度对照磁场。结论成功构建了一套适用于细胞和分子生物学实验的亚磁培养系统,实现了亚磁模拟系统的标准化,对于提高亚磁生物学效应的研究水平有重要的应用价值。Objective To simulate a hypomagnetic field on the ground for providing a platform to investigate its biological effects. Methods A permalloy magnetic shielded box was placed in a cell ineubatorto form the hypomagnetic field. The incubation conditions in the hypomagnetic field and the control magnetic fields were balanced by the temperature-humidity-CO2 control system of the cell incubator and airing fans in the magnetic shielded box. Results The hypomagnetie incubation system provided a 0 -600 nT hypomagnetie environment and a series of control magnetic fields from 9uT to 60 uT. The other incubation conditions were controlled at the same level between the hypomagnetie field and the control magnetic fields. Conclusion A standardized hypomagnetie field simulation system is established. It is suitable to conduct extensive biological experiments under the hypomagnetic condition, such as. seeds, tissues, cells and molecules. It would have important benefit value to improve the study level of biological effects and the counteractive strategies for the negative effects of the hypomagnetie field.

关 键 词：亚磁生物效应 地磁场 亚磁培养系统 坡镆合金 细胞培养 

分 类 号：Q693[生物学—生物物理学]