Wnt5a,Frizzled2及cGMP依赖性蛋白激酶在寻常性银屑病皮损中的表达  

Expression of Wnt5a,Frizzled2 and Cyclic GMP-dependent Protein Kinase in the Skin Lesions of Psoriasis Vulgaris

作　　者：李政霄[1] 涂晨[1] 张燕飞[1] 肖生祥[1] 王娟[1] 郑焱[1] 

机构地区：[1]西安交通大学医学院第二附属医院皮肤科,陕西西安710004

出　　处：《中国皮肤性病学杂志》2013年第7期654-656,669,共4页The Chinese Journal of Dermatovenereology

基　　金：国家自然科学基金(81101197)

摘　　要：目的通过检测寻常性银屑病皮损及正常皮肤中Wnt5a,Frizzled2及cGMP依赖性蛋白激酶的表达,探讨寻常性银屑病新的发病机制。方法用免疫组织化学的方法检测寻常性银屑病及正常组织中Wnt5a,Frizzled2及cGMP依赖性蛋白激酶的表达。结果 Wnt5a及Frizzled2在寻常性银屑病皮损中表达量增加,与正常组织相比差异有统计学意义(P<0.05);而cGMP依赖性蛋白激酶在寻常性银屑病皮损中表达降低,与正常组织相比差异有统计学意义(P<0.05)。cGMP依赖性蛋白激酶在寻常性银屑病皮损中与Wnt5a,Frizzled2的异常表达呈负相关(P<0.05)。结论 Wnt5a及其相关受体Frizzled2参与了银屑病的发病,其下游因子cGMP依赖性蛋白激酶表达被抑制可能是引起角质形成细胞的异常增殖与凋亡,导致银屑病形成的原因。Objective To observe the expression of WntSa, Frizzled2 and cyclic GMP-dependent protein kinase in lesions of psoriasis vulgaris and normal skins. Methods Immunohistochemistry was used to detect expression of WntSa, Frizzled2 and cyclic GMP-dependent protein kinase in normal tissues and psoriatic lesions. Results The positive expression rate of WntSa and Frizzled2 in psoriatic lesions were significantly higher than that in normal skins（P 〈0.05 ）. However, the positive expression rate of cyclic GMP-dependent protein kinase expression in psoriatic lesions were significantly lower than that in normal skins （P 〈 0.05 ）. The expression of cyclic GMP-dependent protein kinase was negatively correlated with the expression of WntSa and Frizzled2 in psoriatic lesions（P 〈 0. 05）. Conclusion WntSa and it＇s related receptors Frizzled2 might be correlated to the pathogenesis of psoriasis. The reduced expression of downstream factor cyclic GMP-dependent protein kinase probably caused the abnormal proliferation and apoptosis of keratinocytes, leading to occurrence of psoriasis vulgaris.

关 键 词：WNT5A Frizzled2 cGMP依赖性蛋白激酶 免疫组织化学 寻常性银屑病 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]