新生儿静脉血与脐带血及孕妇静脉血HBV病原学标记物相关性研究  

The correlation study of HBV serological index in neonate＇s venus, cord blood and mother＇s venus blood

作　　者：易为[1] 张禄雪[2] 胡玉红[1] 李明慧[2] 郝红晓[2] 王士俊[1] 姜秀娟[1] 张书凤[3] 宋淑静[3] 谢尧[1] 梁晓峰[1] 

机构地区：[1]首都医科大学附属北京地坛医院妇产科,北京100015 [2]首都医科大学附属北京地坛医院肝病中心,北京100015 [3]首都医科大学附属北京地坛医院检验科,北京100015

出　　处：《中华实验和临床病毒学杂志》2013年第3期173-176,共4页Chinese Journal of Experimental and Clinical Virology

基　　金："十一五"重大专项:我国乙型病毒性肝炎免疫预防策略研究，首都医学发展基金重点支持项目，国家自然科学基金

摘　　要：目的 探讨慢性HBV感染孕妇所生新生儿脐带血与静脉血HBV标志物状况的一致性和相关性,以及与孕妇HBV感染标志物的相关性.方法 以HBsAg、HBeAg双阳性且HBV DNA＞1 ×105拷贝/ml孕妇及新生儿为研究对象,孕妇分娩前采集静脉血,新生儿于注射乙肝免疫球蛋白、乙肝疫苗前采集静脉血.在清洁和去除脐带表面污染血液,并用酒精消毒后,用注射器采集脐带血.HBsAg、抗HBs、HBeAg、抗HBe采用雅培微粒子化学发光法（美国雅培公司试剂,Abbott Architac i2000）检测,HBV DNA含量经COBAS TagMan HBV DNA定量检测仪检测.结果 共入组孕妇383例及所生新生儿,静脉血和脐带血HBsAg的阳性检出率分别为61.2％和63.9％,HBeAg阳性检出率分别为83.2％和83.5％,HBV DNA阳性检出率分别为56.0％和59.4％,静脉血和脐带血之间均有一致性.静脉血和脐带血间HBsAg、HBeAg和HBV DNA含量的相关性具有统计学意义（r=0.766、0.857、0.692,P＜0.000）.新生儿静脉血和脐带血的HBeAg含量与孕妇的HBeAg含量具有相关性（r=0.362,P=0.000；r=0.352,P=0.000）,而静脉血和脐带血的HBsAg含量与孕妇血清的HBsAg含量无相关性（r=0.023,P=0.785；r=0.04,P=0.604）.结论 慢性HBV感染孕妇所生新生儿脐带血和静脉血HBV标志物状态有良好的一致性,可以以脐带血的HBV标志物反映新生儿静脉血HBV标志物.Objective In this study,we discussed the consistency and correlation of HBV serological indexes between neonates＇ venous blood and cord blood whose mothers had chronical HBV infection,as well as the correlation of thoses indexes with the mothers＇.Method Chronically HBV infected mothers who were postive of both HBsAg and HBeAg and also had a HBV DNA virus load above 105copies/ml and their infants were enrolled.The mothers＇ venous blood were collected before delivery.The neonates＇ cord blood were collected at birth after removal of contaminants and disinfected with alcohol on the cord＇s surface,and the venous blood were collected before hepatitis B virus immune globin（HBIG） and hepatitis B vaccine were given.The levels of HBsAg,anti-HBs,HBeAg and anti-HBeAg were tested with Abbott microparticle chemiluminescence method （Abbott Laboratories,Abbott Architac i2000）.HBV DNA quantification were tested by COBAS TagMan real-time PCR Assay.Results 383 mothers and their infants were enrolled.The positive rates of HBsAg in cord blood and venous blood were 61.2％ and 63.9％.The positive rates of HBeAg level in cord blood and venous blood were 83.2％ and 83.5％.The positive rates of HBV DNA level in cord blood and venous blood were 56.0％ and 59.4％.The state of HBsAg,HBeAg and HBV DNA in cord blood and venous blood were consistency,and significant correlation was observed in their levels with correlation coefficients of 0.766,0.857,and 0.692,respectively （P ＜ 0.000）.Significant correlation of the HBeAg levels were observed between mothers＇ venous blood and neonates＇ venous blood,as well as neonates＇ cord blood with correlation coefficients of 0.362 and 0.352 （P ＜ 0.000）.However,there was no significant correlation of HBsAg levels between them （r =0.023,P =0.785 ; r =0.04,P =0.604）.Conclusions The HBV serological index of neonate＇s cord blood could reflect the HBV serological indexes in venous blood because of the good correlation and consistency between them.

关 键 词：胎血 疾病传播 垂直 肝炎病毒 乙型 肝炎疫苗 乙型 

分 类 号：R512.62[医药卫生—内科学]