人工荨麻疹患者神经肽Y和CD63的检测及意义  

Detection and significance of neuropeptide Y and CD63 in patients with urticaria factitia

作　　者：王爱学[1] 李玉平[1] 王瑞敏[1] 卢凤敏[1] 郭兵申[1] 张环环[1] 石丽萍[1] 姚丽美[1] 四荣联[1] 

机构地区：[1]河北医科大学第二医院皮肤科,石家庄050000

出　　处：《中华皮肤科杂志》2013年第7期513-514,共2页Chinese Journal of Dermatology

摘　　要：目的探讨神经肽Y和活化的嗜碱性粒细胞在人工荨麻疹发病机制中的意义。方法人工荨麻疹患者24例来自2011年8—12月河北医科大学第二医院皮肤科门诊，16例对照为除外过敏性疾病、躯体慢性疾病、神经系统及精神疾病的同期健康志愿者。用放射免疫分析法检测血浆神经肽Y的表达；用CD45-PERCP（多甲藻黄素-叶绿素-蛋白质复合物）、CD203c-藻红蛋白、CD63-异硫氰酸荧光素三联抗体标记嗜碱性粒细胞，流式细胞仪检测CD63的表达水平，计数活化嗜碱性粒细胞数量。结果人工荨麻疹患者与健康对照组神经肽Y的含量分别为（111．155±36．832）n#L和（104．456±44．697）ng/L，两组差异无统计学意义（t=0．198，P〉0．05）；人工荨麻疹患者外周血活化嗜碱性粒细胞中位数（280．5×10^6／L）高于健康对照组（18．1×10^6/L），两组差异有统计学意义（P〈0．05）。结论活化的嗜碱性粒细胞可能参与了人工荨麻疹的发病过程，而神经肽Y的作用尚不能确定。Objective To investigate the role of neuropeptide Y and activated basophilic granulocytes in the pathogenesis of urticaria faetitia. Methods This study included 24 outpatients with urticaria factitia collected from August 2011 to December 2011 at the Department of Dermatology, Second Hospital of Hebei Medical University, as well as 16 healthy volunteers without allergic diseases, chronic somatic diseases, nervous system disorders or psychiatric disorders. Venous blood samples were collected from these subjects. Radioimmunoassay was carried out to determine the plasma level of neuropeptide Y, and flow eytometrie analysis to count activated basophilic granulocytes after triple staining with CD45-peridinin-chlorophyll-protein complex/CD203c- phycoerythrin/CD63-fluorescein isothioeyanate. Results The plasma level of neuropeptide Y was （111.155 ± 36.832） ng/L and （104.456 ± 44.697） ng/L in the patients and healthy volunteers respectively （t = 0.198, P 〉 0.05）. The median number of activated basophilic granulocytes was significantly higher in the patients than in the healthy controls （280.5 × 10^6/L vs. 18.1 × 10^6/L, P 〈 0.05）. Conclusisons Activated basophilie granuloeytes appear to be involved in the pathogenesis of urticaria factitia, while the role of neuropeptide Y remains unknown.

关 键 词：血浆神经肽Y 人工荨麻疹 CD63 患者 检测 嗜碱性粒细胞 河北医科大学第二医院 放射免疫分析法 

分 类 号：R758[医药卫生—皮肤病学与性病学]