宫内香烟烟雾暴露对新生大鼠延髓3-巯基丙酮酸转硫酶表达的影响  

Effects of Intrauterine Cigarette Smoking Exposure on Expression of 3-mercaptopyruvate Sulfurtransferase in Medulla Oblongata of Neonatal Rats

作　　者：聂黎虹[1,2] 胡雅洁[1] 李显科[1] 薛莲[1] 贾清怡[3] 杨以文[1] 郑煜[1] 

机构地区：[1]四川大学华西基础医学与法医学院生理学教研室,成都610041 [2]宁夏医科大学基础医学院生理学系,银川750004 [3]四川大学华西临床学院,成都610041

出　　处：《四川大学学报（医学版）》2013年第4期526-530,共5页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(No.31271233;J1103604);教育部博士点基金(No.20100181110048;20120181110094)资助

摘　　要：目的探讨3-巯基丙酮酸转硫酶(3-mercaptopyruvate sulfurtransferase,3MST)在正常新生大鼠延髓的表达及宫内香烟烟雾暴露对其表达的影响。方法 SD孕鼠随机分为正常对照组和香烟烟雾暴露组(n=8),与孕鼠相对应的新生(1d)大鼠为本研究的实验对象。采用RT-PCR和Western blot法,分别观察正常新生大鼠延髓组织中3MST mRNA和蛋白的表达情况及宫内香烟烟雾暴露对其表达的影响;采用免疫组织化学法观察正常新生大鼠延髓呼吸相关核团神经元3MST的表达和分布情况及宫内香烟烟雾暴露对其表达的影响。结果正常对照组新生大鼠延髓组织中有3MST mRNA和蛋白的表达;香烟烟雾暴露组新生大鼠延髓组织中3MST mRNA和蛋白的表达水平显著增加(P<0.05)。3MST在正常对照组新生大鼠前包钦格复合体(pre-BtC)、舌下神经核(12N)、疑核(Amb)、面神经核(FN)和孤束核(NTS)神经元中有弱表达。与对照组比较,香烟烟雾暴露组新生大鼠pre-BtC、12N、Amb和FN神经元中3MST免疫阳性产物平均光密度值增加(P<0.05);NTS神经元中3MST免疫阳性产物平均光密度值无明显变化(P>0.05)。结论 3MST存在于正常新生大鼠延髓呼吸相关核团神经元;宫内香烟烟雾暴露可上调延髓3MST的表达,提示3MST-硫化氢(H2S)途径可能参与宫内香烟烟雾暴露致新生大鼠延髓呼吸中枢损伤保护过程。Objective To investigate the expression of 3-mercaptopyruvate sulfurtransferase （3MST） in medulla oblongata of neonatal rats and effects of intrauterine cigarette exposure on its expression. Methods Sprague Dawley pregnant rats were randomly divided into 2 groups, control group and cigarette smoke exposure group （n= 8）. 3MST mRNA and protein expression in medulla oblongata of neonatal rats were analysed by RT-PCR and Western blot, respectively, and the expression of 3MST in the neurons of respiratory-related nuclei in medulla oblongata of neonatal rats was investigated with immunohistochemical technique. Results The RT-PCR and Western blot analyses showed that 3MST mRNA and protein were expressed in the medulla oblogata of neonatal rats and intrauterine cigarette exposure promoted their expression （P〈0.05）. Immunohistochemical staining indicated that 3MST existed in the neurons of pre-Botzinger complex （pre-BotC）, hypoglossal nucleus （12N）, ambiguous nucleus （Amb）, facial nucleus （FN） and nucleus tractus solitarius （NTS） in control group of the animals and the mean optical densities of 3MST-positive neurons in the pre-BotC, 12N, Amb and FN, but not NTS, were significantly increased in cigarette smoke exposure group （P〈0.05）. Conclusions 3MST exists in the neurons of medullary respiratory nuclei of neonatal rats and its expression can be up-regulated by intrauterine cigarette exposure, suggesting that the 3MST H2S pathway may be involved in protection of medullary respiratory centers against injury induced by intrauterine cigarette exposure.

关 键 词：3-巯基丙酮酸转硫酶 香烟烟雾 硫化氢 延髓 呼吸中枢 

分 类 号：R714.2[医药卫生—妇产科学]