刹毒草合剂中黄芪甲苷的含量测定研究  

Determination of Astragaloside in Shaducao Mixture by HPLC

作　　者：叶挺梅[1] 李庆利 王英秀 王智威 卢舜飞[1] 

机构地区：[1]丽水学院,浙江丽水323000 [2]浙江省丽水市安福生物科技有限公司,浙江丽水323000

出　　处：《中国民族民间医药》2013年第11期10-10,12,共2页Chinese Journal of Ethnomedicine and Ethnopharmacy

基　　金：浙江省科技厅重大科技专项社会发展项目(2011C03002)

摘　　要：目的:建立测定刹毒草合剂中黄芪甲苷的高效液相色谱方法。方法:采用Diamonsil(钻石)C18(250×4.6mm,5(m)色谱柱;流动相为乙腈-水(33∶67);流速:1.0ml/min;柱温:35℃;漂移管温度为105℃,气体流速为2.7L/min。结果:黄芪甲苷在1.04～5.2μg范围内线性关系良好,平均回收率为101.4%,RSD为1.24%(n=6)。结论:该方法操作快速,结果准确,可作为刹毒草合剂的质量控制方法之一。Objective： To establish an HPLC method for determination of Astragaloside in Shaducao Mixture. Method： The es- tablish an HPLC method for determination of astragaloside in in Shaducao Mixture. Method： HPLC was carried out on the Diamonsil C18 column （250 × 4. 6mm, 5 （m） with acetonitrile - water （33：67 ） at 35 ℃, the flow rate was 1.0 mL/min; Drift tube tempera- ture was 105℃, gas flow rate was 2. 7L/min. Result： The calibration curves was linear in the ranges of 1.04 -5.2μg. The average recovery was 101.4% with RSD 1.24% （n =6）. Conclusion： The method is rapid and simple, it can be used for quality control of Shadueao Mixture.

关 键 词：刹毒草合剂 黄芪甲苷 高效液相色谱 含量测定 

分 类 号：R284.1[医药卫生—中药学]