两种中药复方对脑缺血再灌注大鼠脑组织Glu含量及NMDA受体亚单位NR1表达的影响  

Comparative Study on Effects of Two Traditional Chinese Medicinal Compounds on Content of Glutamate and Expressions of NR1 in Cerebral Tissue of Rats after Focal Cerebral Ischemia and Reperfusion

作　　者：王业梅[1] 胡建鹏[1] 程惠娟[1] 

机构地区：[1]安徽中医药大学,安徽合肥230038

出　　处：《中国民族民间医药》2013年第12期33-34,共2页Chinese Journal of Ethnomedicine and Ethnopharmacy

基　　金：国家自然科学基金面上项目(81173629);安徽省自然科学基金课题(1208085QH150)

摘　　要：目的:比较益气活血汤、镇肝熄风汤和星蒌承气汤3种中药复方对急性脑缺血再灌注大鼠缺血脑组织Glu含量与NR1表达的影响。方法:采用线栓法阻断大鼠左侧大脑中动脉制作局灶性脑缺血再灌注模型。大鼠随机分为假手术组、模型组、镇肝熄风汤和星蒌承气汤组。采用高效液相色谱法和免疫组化SABC染色法,分别检测Glu的含量和NR1表达。结果:脑缺血2h再灌注各时间点与假手术组比较,模型组Glu含量和NR1表达均显著升高和增加(P<0.05或P<0.01);与模型组比较,各治疗组各时间点Glu含量和NR1表达显著降低和减少(P<0.01);治疗组组间比较,48h无显著差异,6h星蒌承气汤组在减少NR1表达方面最优(P<0.05);结论:两种中药复方可以通过降低Glu含量和减少NR1表达,减轻兴奋性氨基酸毒性,抗脑缺血再灌注损伤。Objective： To compare the effects of two traditional Chinese medicinal compounds on content of glutamate and expres- sions of NR1 in cerebral tissue of rats after focal cerebral ischemia and reperfusion （I/R） . Methods： The local cerebral I/R model was established by ligation of the middle cerebral arteries （MCA） . The animals were divided into the sham - operative group, the model group, the Zhengan Xifeng Decoction （ZXD） group and the Xinglou Chengqi Decoction （XCD） group. The contents of gluta- mate was detected by HPLC. Im -munohistochemical SABC method was used to obsevre the expressions of NR1. Rdusts： Compared with-those of the sham- operative group, the contents of glutamate and expressions of NR1 increased markedly at all time pionts after I/R in model group. Compared with those of model group, the contents of glutamate and expressions of NR1 decreased or reduced at all time pionts after I/R in all treatment groups. The optimal effects were shown in XCD at 6 h in reducing expressions of NR1. Con- clusion： The two traditional Chinese medicinal compounds could reduce pathologic injury after focal cerebral I/R by decreasing or re- ducing contents of glutamate and expressions of NR1.

关 键 词：脑缺血再灌注 镇肝熄风汤 星蒌承气汤 谷氨酸 N-甲基-D-天冬氨酸受体 

分 类 号：R277.733.1[医药卫生—中医学]