人工合成人恶性疟原虫58肽抗原基因在大肠杆菌中的表达  

Expression of a synthetic gene of plasmodium falciparum antigen in escherichia coli

作　　者：马维芳[1] 金福军 钟雄林[1] 陈仕荣[1] 王昌才[1] 王启松[2] 闵永洁 

机构地区：[1]第一军医大学生物化学教研室 [2]上海市复旦大学遗传研究所

出　　处：《第一军医大学学报》1991年第2期109-113,共5页Journal of First Military Medical University

基　　金：广东省自然科学基金资助

摘　　要：使用DNA合成仪合成的人恶性疟原虫58肽抗原基因,与大肠杆菌表达性载体质粒PWR450—Ⅰ重组,转化到大肠杆菌JM83中。用斑点杂交技术和电泳分析DNA方法筛选出阳性重组转化菌株,经SDS-聚丙烯酰胺凝胶蛋白电泳分析,证明合成基因在大肠杆菌中表达,产生与β—半乳糖苷酶融合的多肽。A gene of Plasmodium falciparum coding for 58 amino acids ( P. falciparum gene B ) was synthesized with a DNA synthersizer and was recombined with an expression vector PWR450- I , a plasmid of E. coli. The positive recombinants were screened by dot hybridization techique, DNA gel eletrophoresis and protern SDS-polyacrylamide gel eletrophoresis, the results showed that the synthetic gene was expressed in E. coli, and the product was a fusion protein of galactosidase and the gene product.

关 键 词：DNA合成 恶性疟原虫 抗原基因 

分 类 号：R382.31[医药卫生—医学寄生虫学]