外周血T淋巴细胞亚群的变化及其与慢性乙型肝炎的相关性研究  

The changes of peripheral blood T-lymphocyte subsets and their correlation with chronic hepatitis B

作　　者：江浪进[1] 

机构地区：[1]中国人民解放军第二〇二医院检验科,辽宁沈阳110003

出　　处：《国际检验医学杂志》2013年第11期1370-1371,共2页International Journal of Laboratory Medicine

摘　　要：目的监测外周血T淋巴细胞亚群中CD3+、CD4+及CD8+细胞在慢性乙型肝炎患者和健康人群间的变化,研究慢性乙型肝炎患者在细胞免疫方面的动态。方法用流式细胞仪监测外周血CD3+、CD4+及CD8+细胞数量变化,用全自动生化分析仪检测血清中丙氨酸氨基转移酶(ALT)。结果慢性乙型肝炎患者外周血CD3+、CD4+细胞数及CD4+/CD8+比值明显低于健康人群(P<0.01),而CD8+细胞数明显高于健康人群(P<0.01)。慢性乙型肝炎患者中血清转氨酶异常者外周血CD3+、CD8+细胞数明显升高(P<0.01);CD4+细胞数略有升高,但无统计学意义(P>0.05),CD4+/CD8+比值明显下降(P<0.05)。结论慢性乙型肝炎患者T淋巴细胞的改变,可导致细胞免疫功能改变,使得HBV难以清除;慢性乙型肝炎患者CD8+细胞数与血清转氨酶浓度有平行关系。Objective To monitor the changes of peripheral blood CD3＋,CD4＋ and CD8＋ lymphocyte subsets between chronic hepatitis B patients and healthy people,and further to investigate the dynamic variations of cellular immunities of hepatitis B patients.Methods Flow cytometry（FACS） was used to count the numbers of CD3＋,CD4＋ and CD8＋ lymphocytes.The concentrations of alanine transaminase（ALT） in serum were detected by automatic biochemistry analyzer.Results The numbers of CD3＋,CD4＋ lymphocytes and CD4＋/CD8＋ ratio values of chronic hepatitis B patients were significantly lower than those of healthy people（P0.01）.However,the CD8＋ cell numbers of chronic hepatitis B patients were much higher than healthy people（P0.01）.In chronic hepatitis B patients with abnormal ALT,the numbers of CD3＋ and CD8＋ cells were remarkably increased（P0.01）,and CD4＋ lymphocytes increased lightly（with no significance）.However,the CD4＋/CD8＋ ratio values of these patients decreased remarkably.Conclusion The changes of numbers and ratio values of T-lymphocytes will induce the cellular immunities changes,which further improve the activity of HBV.Furthermore,there is positive correlation between the counts of CD8＋ lymphocytes of hepatitis B patients and the concentration of alanine transaminase.

关 键 词：慢性乙型肝炎 T淋巴细胞亚群 谷氨酸氨基转移酶 

分 类 号：R512.62[医药卫生—内科学]