复发性流产患者注射免疫球蛋白对NK细胞的影响  

Impact of intravenous immunoglobulin on peripheral blood natural killer cells in women with recurrent spontaneous abortion

作　　者：滕奔琦[1] 张媛[1] 魏方[1] 李玲[1] 范建辉[1] 

机构地区：[1]中山大学附属第三医院妇产科,广州510630

出　　处：《国际医药卫生导报》2013年第13期1899-1901,共3页International Medicine and Health Guidance News

基　　金：广东省科技计划项目（2009B060700032）

摘　　要：目的 探讨复发性流产（recurrent spontaneous abortion,RSA）患者静脉滴注免疫球蛋白对其外周血NK细胞的影响.方法 2011年1月至2012年6月在我院住院的RSA患者共38例.其中18例静脉滴注免疫球蛋白（IVIG）治疗,作为研究组；20例不同意使用免疫球蛋白治疗的RSA患者作为对照组.比较两组患者外周血NK细胞毒性及NK细胞在所有淋巴细胞中的比例.结果 治疗前,研究组NK细胞毒性为（22.4±7.9）％,NK细胞比例为（25.1±9.4）％；治疗后,研究组NK细胞毒性为（15.0±4.2）％,NK细胞比例为（26.2±8.7）％.第一次检测时,对照组NK细胞毒性为（21.7±8.1）％,NK细胞比例为（24.0±9.0）％；间隔10d后复测,对照组NK细胞毒性为（25.6±7.9）％,NK细胞比例为（23.6±6.8）％.静脉滴注免疫球蛋白后,研究组患者静脉外周血NK细胞毒性显著降低,差异有统计学意义（P＜ 0.05）,但NK细胞的比例无显著变化,差异无统计学意义（P＞0.05）.对照组的第1次与第2次外周血NK细胞毒性比较差异无统计学意义（P＞ 0.05）,2次NK细胞的比例比较差异亦无统计学意义（P＞0.05）.经过住院安胎治疗后,研究组妊娠时间超过24周的占88.9％（16/18）,对照组超过24周的占55.0％（11/20）,两组相比差异有统计学意义（P＜ 0.05）.结论 RSA患者静脉滴注免疫球蛋白可降低NK细胞毒性,提高RAS患者的安胎成功率.Objective To explore the impact of intravenous immunoglobulin on peripheral blood natural killer （NK） cells in women with recurrent spontaneous abortion （RSA）.Methods Of 38 patients with RSA who had been hospitalized during the period of January 2011 to June 2012,18 patients received intravenous immunoglobulin （study group） while the rest 20 who refused to receive immunoglobulin were assigned to a control group.Peripheral NK cell cytotoxicity and rate of NK cells in all types of lymphocytes were detected before and after treatment by flow cytometry.Results NK cell cytotoxicity was（22.4 ＋ 7.9）% in the study group and （21.7 ± 8.1）% in the control group before treatment,was （15.0 ± 4.2）% in the study group and （25.6 ± 7.9）% in the control group after treatment.Before treatment,the rate of NK cell was （25.1 ± 9.4） % in the study group and （24.0 ± 9.0） % in the control group,while after treatment it was （26.2 ± 8.7） % in the study group and （23.6 ± 6.8） % in the control group.NK cell c.vtotoxicity but not the rate of NK cell was significantly reduced （P〈0.05） in the study group.After treatment,NK cell cytotoxicity and the rate of NK cell did not differ significantly （P〉0.05）.88.9% of the patients in the study group but 55.0% of the patients in the control group had pregnancy duration of longer than 24 weeks after tocolytic treatment （P〈0.05）.Conclusions Intravenous immunoglobulin can reduce NK cell cytotoxicity,leading to an increase in the success rate of miscarriage prevention in patients with recurrent spontaneous abortion.

关 键 词：复发性流产 静脉滴注免疫球蛋白 NK细胞 

分 类 号：R714.21[医药卫生—妇产科学]