猪附红细胞体MSG1基因的克隆及序列分析  

Cloning and Sequence Analysis of MSG1 Gene of Mycoplasma suis

作　　者：曹薇[1] 周鹏[1] 米荣升[1] 黄燕[1] 秦培兰[1] 杨晓娇[1] 王向佩[1] 王晓娟[1] 石凯[1] 陈兆国[1] 

机构地区：[1]中国农业科学院上海兽医研究所农业部动物寄生虫学重点开放实验室中国农业科学院动物源性食品安全研究中心,上海200241

出　　处：《动物医学进展》2013年第7期44-48,共5页Progress In Veterinary Medicine

基　　金：公益性行业(农业)科研专项项目(200903036-05)

摘　　要：为研究猪附红细胞体MSG1基因的遗传变异特点,从上海地区3个屠宰场采集自然感染附红细胞体的猪血液,提取基因组DNA,根据GenBank已经发表的猪附红细胞体MSG1基因序列(登录号AM407404.1),设计引物进行PCR扩增,将扩增产物连接到pMD18-T载体上,进行序列测定和分析。结果共获得了82条猪附红细胞体MSG1基因序列,序列长度为1 011bp,共编码336个氨基酸。同源性分析显示与GenBank中已经发表的猪附红细胞体MSG1基因核苷酸序列相似性为96.5%～98.3%,氨基酸的相似性为97.9%～99.1%。MSG1基因核苷酸序列的系统进化分析表明,上海地区猪附红细胞体分离株与GenBank登录的德国54/96分离株序列的亲缘关系较远。In order to study the genetic variation of MSG1gene of Mycoplasma suis,fresh blood of pigs infected with M.suis were collected from 3slaughterhouses in 3districts of Shanghai,and their genomic DNA were extracted.The MSG1genes of M.suis encoding putative adhesin G1protein were amplified by PCR with a pair of specific primers and the PCR products were inserted into vector pMD18-T.The recombinant plasmids were transformed into Escherichia coli DH5αand sequenced.The sequences of MSG1gene were analyzed and compared with that of the NCBI published strain.The results showed that 82full length of MSG1genes were sequenced successfully and the nucleotide sequence length of MSG1gene were 1 011bp,coding 336amino acid residues.Homology analysis showed that the nucleotide sequence identities compared with the NCBI published strain ranged from 96.5%-98.3%and 97.9%-99.1%for deduced amino acid sequence.Phylogenetic analysis revealed that the hereditary distance of the Shanghai isolates was far from that had been deposited in GenBank.

关 键 词：猪附红细胞体 MSG1基因 克隆 序列分析 

分 类 号：S852.62[农业科学—基础兽医学]