三种抗球虫药物对家兔CYP1A2 mRNA和蛋白表达的影响  

Effect of Three Different Coccidiostats on Expression of CYP1A2 mRNA and Protein in Rabbits

作　　者：刘春[1,2] 吴刘成[2] 王胜洁[2] 邵义祥[2] 

机构地区：[1]扬州大学兽医学院,江苏扬州225009 [2]南通大学实验动物中心,江苏南通226001

出　　处：《动物医学进展》2013年第7期69-73,共5页Progress In Veterinary Medicine

基　　金：南通市科技创新计划(AS2010020);南通大学研究生科技创新计划(YKC13053)

摘　　要：利用Real-time PCR和Western blot分别检测抗球虫药物磺胺喹噁啉、氯苯胍和妥曲珠利对家兔肝脏、肾脏、十二指肠中CYP1A2mRNA和蛋白表达的影响。40只新西兰白兔分为空白对照组、磺胺喹噁啉处理组、氯苯胍处理组和妥曲珠利处理组。空白对照组家兔饲喂正常颗粒饲料,磺胺喹噁啉处理组饲喂含25mg/kg浓度磺胺喹噁啉的颗粒饲料,氯苯胍处理组饲喂含66mg/kg浓度氯苯胍的颗粒饲料,妥曲珠利处理组饲喂含2mg/kg浓度妥曲珠利的颗粒饲料,连续30d。在第31天无菌条件下取出动物的部分肝脏、肾脏以及十二指肠,提取RNA、蛋白分别进行Real-time PCR、Western blot检测。Real-time PCR的结果表明,磺胺喹噁啉、氯苯胍和妥曲珠利都能够诱导家兔肝脏和肾脏CYP1A2mRNA的表达,而妥曲珠利能够显著的抑制十二指肠CYP1A2mRNA的表达。Western blot结果表明,磺胺喹噁啉、氯苯胍和妥曲珠利能够显著的诱导家兔肝脏CYP1A2蛋白水平的表达;磺胺喹噁啉显著的诱导肾脏和十二指肠CYP1A2蛋白表达,而氯苯胍能够显著的抑制肾脏CYP1A2蛋白的表达。结果提示,磺胺喹噁啉、氯苯胍和妥曲珠利对家兔CYP1A2mRNA和蛋白水平的表达均具有组织特异性,在不同的组织器官呈现出显著的诱导和抑制作用。We investigated the action of coccidiostats such as sulfaquinoxaline（SUL）,robenidine（ROB）and toltrazuril（TOL）on CYP1A2mRNA and protein in liver,kidney and duodenum of rabbit by real-time PCR and Western blot technology.40adult health female or male New Zealand white rabbits were randomly divided into 4 groups：control group（n=10）,25mg/kg SUL group（n=10）,66mg/kg ROB group（n=10）,and 2mg/kg TOL group（n=10）,the rabbits in groups were respectively fed with pellet feed containing the dugs of specific concentration for 30days.On the thirtieth day,the liver,kidney and duodenum were removed to keep germ free from each group rabbits,and the total RNA and protein were extracted from each tissue for real-time PCR and Western blot detection.The result of real-time PCR indicated the expression of CYP1A2mRNA could be induced in liver and kidney by SUL,ROB and TOL,in contrast it be inhibited in duodenum by TOL.The results indicated that the tissue specificity expressions of CYP1A2mRNA and protein in rabbits by SUL,ROB and TOL,and the significant effect of induction and inhibition in different tissues and organs were found.

关 键 词：磺胺喹噁啉 氯苯胍 妥曲珠利 家兔 CYP1A2 

分 类 号：S852.723[农业科学—基础兽医学] Q789[农业科学—兽医学]