聚合酶链反应检测乙型肝炎病毒核酸的实验研究  

Detection of HBV DNA by polymerase chain reaction-An experimental study

作　　者：周荣[1] 梁炽森[1] 骆抗先[1] 

机构地区：[1]南方医院传染病科

出　　处：《第一军医大学学报》1991年第3期185-188,共4页Journal of First Military Medical University

摘　　要：以国产DNA聚合酶和试剂建立聚合酶链反应,检测乙型肝炎病毒DNA,如以溴乙啶荧光显带可检出1fg HBV DNA,继以分子杂交则至少可检出0.1fg,灵敏性与国外报告相近。两组引物可分别获得208bp和415bp的扩增分子,与按序列计算的结果一致,并可与HBV DNA探针杂交,表明反应的特异性。试用于检测20例慢性活动性肝炎病人血清,检出HBV DNA17例,而斑点杂交仅可检出6例。A Polymerase chain reaction has been developed to detect HBV DNA using FD DNA polymerase and domestic reagents. One fg of HBV DNA can be detected if the PCR product is electrophoresed and stained with ethidine bromide; 100ag if hybridized after Southern blotting. So the sensitivity of the test is identical with that reported abroad. Amplified number of DNA molecules of 205bp and 413 bp are derived from each set of primers respetively, and are consistent with the results calculated from the sequence. Besides,they can be hybridized with the HBV probe. Those suggest the reaction is specific. It was attempted to test the sera from 20 patients with chronic active hepatitis. HBV DNA was detected in 17 cases while only in 6 with spot hybridization.

关 键 词：聚合酶链反应 乙肝病毒 核酸 

分 类 号：R512.620.4[医药卫生—内科学]