中华鳖小肠出血病的组织病理学诊断  

Histopathologic Diagnosis of Small Intestinal Hemorrhagic Disease in Trionyx sinensis

作　　者：牟群[1] 徐培培[1] 潘连德[1] 

机构地区：[1]上海海洋大学省部共建水产种质资源发掘与利用教育部重点实验室,上海201306

出　　处：《动物医学进展》2013年第7期121-126,共6页Progress In Veterinary Medicine

基　　金：国家自然科学基金项目(39970582);上海市教育委员会重点项目(07ZZ135);上海市重点学科建设项目资助(Y1101);上海海洋大学研究生科研基金项目

摘　　要：通过组织病理观察对具有明显白底板症状的病鳖进行了组织病理学诊断。结果表明,病鳖胃壁结构较完整,胃腺腺细胞稍肿大,首次在胃贲门和幽门黏膜层发现有大量嗜酸性粒细胞。肠出血严重,前、中肠段均有出血,肠黏膜上皮脱落、坏死;前肠壁各层可见大量含铁血黄素沉着;中肠黏膜层可见大量红细胞渗出,固有膜和黏膜下层血管肿胀,淤积大量血细胞,并可见大量白细胞浸润,严重者结构消失;后肠黏膜层萎缩,肠绒毛缩短,肠壁变薄,其他组织器官未见明显出血。肾小球严重萎缩、坏死,肾小管上皮细胞肿大、变性,并向管腔靠近,甚至与基膜剥离,严重者结构消失。肝细胞肿胀,窦状隙扩张、淤血;脾脏被膜增厚、红髓区白细胞增多,白髓区淋巴细胞减少,大量白细胞变性、坏死,血管管腔及周围有大量红细胞。鉴此,依据病鳖主要病变为肠出血,该病诊断为具有"白底板"症状的中华鳖肠出血病。本研究结果为中华鳖肠出血病的临床诊断提供了理论依据。In this study,histopathologic diagnosis in Chinese soft-shelled turtles with the typical white abdominal shell（WAS）symptom was made.The histological findings indicated that the histological structure of the stomach was not disturbed,but the swollen gastric gland cells,besides,agreat deal of eosinophil infiltration was visible both in the lamina propria and the submucosa layer of cardia and the pylorus.It also showed that the intestine was damaged most seriously,and obvious hemorrhage in different parts of the foregut and midgut was observed.Local necrosis occurred in the intestinal mucosa,accompanied by mucosal epithelial cells shedding and necrosis.Large amount of hemosiderosis occurred in different layers of the foregut.In midgut,agreat deal of red blood cells（RBC）exuded in the mucosa layer.Vascular swelling occurred in lamina propria and submucosa,in which large amounts of RBC deposition accompanied by neutrophil infiltration have been found.Notably,the structure of the mucosa layer has disappeared.In hindgut,intestinal mucous membrane atrophy and shorten intestinal villus were detected,but hemorrhage in other organs was not obvious.Alterations in the kidney included serious atrophy and necrosis of the glomerular,most epithelial cells of the tubule along with the nucleus swelling and microvilli necrosis were visible,moreover,epithelial cells were approached to the cavity.The hepatocyte was swollen,sinusoidal dilation and congestion were also discovered.Leukocytosis in the red pulp was increased,but leukocytes in the white pulp were decreased,even worse,degeneration and necrosis of a large number of the leukocytes in the white pulp were found.Besides,a large number of RBCs were found in the vascular lumen and the surrounding.In view of this,it was diagnosed as small intestinal hemorrhagic disease with white abdominal shell symptoms in Trionyx sinensis.The study provided the theoretical basis of the clinical diagnosis.

关 键 词：中华鳖 肠出血病 组织病理学 

分 类 号：S852.31[农业科学—基础兽医学] S947.1[农业科学—兽医学]