我国几个不同地域长梗扁桃苗木的抗寒性研究  

Cold-resistance of Amygdalus pedunculata from Different Provenances in China

作　　者：郭改改[1] 魏钰[1] 封斌 麻保林 张应龙 郭春会[1] 

机构地区：[1]西北农林科技大学园艺学院,陕西杨陵712100 [2]陕西省治沙研究所,陕西榆林719000 [3]神木县生态协会,陕西神木719300

出　　处：《西北林学院学报》2013年第4期11-15,230,共6页Journal of Northwest Forestry University

基　　金：国家林业局林业公益性行业科研专项(201104074);西北农林科技大学唐仲英育种专项

摘　　要：分别取自河北、固阳、神木、榆林、乌审旗5地生长状况基本相同的长梗扁桃1年生休眠枝条为材料,通过人工冷冻模拟外界环境,对长梗扁桃枝条进行-15℃、-19℃、-23℃、-27℃、-31℃、-35℃、-39℃等7个温度梯度处理。测得这些休眠枝条的相对电导率、SOD、POD活性、游离脯氨酸含量及MDA含量,并对低温处理过的部分枝条进行恢复生长来测定其萌芽率,最后对测定结果进行分析和比较。结果表明:5个地区的长梗扁桃的相对电导率、SOD、POD活性、游离脯氨酸含量及MDA含量均随着处理温度的降低而呈现上升趋势,并且抗寒性强的地区的长梗扁桃SOD、POD活性和游离脯氨酸含量相对较高,MDA含量和电导率较低,恢复生长枝条的萌芽率均比同温度处理下的抗寒性弱的高。抗寒性强弱顺序为:河北(H)>乌审旗(W)>固阳(G)>神木(S)>榆林(Y),河北和乌审旗的长梗扁桃抗寒性较强,可以对其进行广泛的品种选育和推广。The annual dormant Amygdalus pedunculata branches were collected from five different provenances（Hebei,Guyang,Shenmu,Yulin,and Wushenqi） in China to examine their cold resistance.The branches were treated under 7 artificial simulated low temperatures：-15℃,-19℃,-23℃,-27℃,-31℃,-35℃,and-39℃.Relative parameters of the branches subject to cold treatment were measured,including the relative leakage of electrolytes,super oxide dismutase（SOD） activity,peroxidase（POD） activity,the contents of proline and malondialdehyde（MDA）.The recovery rates of germination of the treated branches were examined and compared.The values of the parameters all increased with the decrease of temperature.The species with excellent cold resistance had higher contents of proline,higher activity of SOD and POD,but lower MDA and relative leakage of electrolytes.After the recovery from low temperature,the species with excellent cold resistance had a significantly higher germinating rate than the other species.The sequence of cold resistance was in the order of Hebei（H）Wushenqi（W）Guyang（G）Shenmu（S） Yulin（Y）.Both Hebei and Wushenqi showed excellent cold resistance,and can be widely introduced and extended.

关 键 词：长梗扁桃 抗寒性 相对电导率 保护酶系统 脯氨酸 丙二醛 恢复萌芽率 

分 类 号：S662.9[农业科学—果树学]