乙酰丁香酮对不同糯玉米受体系统遗传转化的影响  

Effect of Acetosyringone on Genetic Transformation of Receptor System of Different Waxy Maizes

作　　者：郭新梅[1,2] 车昕明[1] 裴玉贺[1] 宋希云[1] 

机构地区：[1]青岛农业大学农学与植物保护学院,山东青岛266109 [2]山东登海种业股份有限公司/山东省玉米育种与栽培技术企业重点实验室,山东莱州261448

出　　处：《西南农业学报》2013年第3期899-902,共4页Southwest China Journal of Agricultural Sciences

基　　金："十二五"国家科技支撑计划(2011BAD35B01);山东省泰山学者岗位(作物栽培与育种学);山东省现代农业产业技术体系创新团队项目;青岛市科技计划基础研究项目(12-1-4-5-(11)-jch);青岛农业大学高层次人才启动基金项目(630740);山东省玉米育种与栽培技术企业重点实验室

摘　　要：乙酰丁香酮(AS)对农杆菌介导的遗传转化有着重要的影响,为提高糯玉米遗传转化率,本试验以莱农糯38的茎尖和幼胚愈伤组织为受体材料,比较悬浮培养基的pH,乙酰丁香酮添加方式及浓度对糯玉米遗传转化的影响。结果表明:悬浮培养基pH为5.2,在伤口处滴加2μl浓度为150μmol/L的AS能显著提高糯玉米茎尖转化的效率。玉米愈伤转化中,在侵染液中添加5 mg/L的AS能显著提高抗性愈伤率。Acetosyringone（AS） plays an important role on maize genetic transformation of the agrobacterium-mediated genetic transformation system.In order to improve the transformation frequency of waxy maize,taken the callus and shoot tips of Lainongnuo 38 as receptors materials,the effects of pH value of infection medium,adding ways and dosages of AS on the genetic transformation of waxy maize were studied.The results showed that when pH value was 5.2 in infection medium,dropping 2 μl AS（with a concentration of 150 μmol/L） on the wound of shoot tips,the transformation frequency of shoot tips was significantly enhanced,while during callus transformation,adding 5 mg/L AS to infection medium of callus,the frequency of resistant callus was significantly enhanced.

关 键 词：受体系统 乙酰丁香酮 添加方式 转化率 

分 类 号：S513[农业科学—作物学]