四川辣椒炭疽病菌鉴定及育种材料抗性筛选  

Identification of Pepper Anthracnose and Resistant Screen of Breeding Materials in Sichuan

作　　者：张国芝[1,2] 赵霞[3] 杨海艳[2] 吴婕[1] 王云月[2] 彭化贤[1] 

机构地区：[1]四川省农业科学院植物保护研究所,四川成都610066 [2]云南农业大学植物保护学院,云南昆明650201 [3]四川省农药检定所,四川成都610041

出　　处：《西南农业学报》2013年第3期1026-1029,共4页Southwest China Journal of Agricultural Sciences

基　　金：四川省财政厅"蔬菜品种抗病性评价与病虫害绿色防控技术"项目;公益性行业(农业)科研专项经费干制辣椒(200903025);国家现代农业产业技术体系四川蔬菜创新团队项目;农业部西南作物有害生物综合治理重点实验室

摘　　要：四川省西充县发现1种严重危害辣椒果实的辣椒炭疽病,造成部分田块绝收。田间调查发现,此病菌既能危害未熟果也能危害红熟果,但未危害叶片或茎干。在果实上病斑圆形,长椭圆形或不规则。在实验室PSA培养基上菌落圆形,边缘整齐,粉红色或橙色,无孢子堆,无菌核,分生孢子盘无刚毛。分生孢子单生,梭形,大小11.703～13.657μm×3.074～4.773μm,附着胞圆形或长椭圆形,褐色,大小为3.985～6.633μm×3.954～6.170μm。菌株rDNA ITS的PCR产物经测序后进行BLAST比对分析,结果显示,此菌株的ITS序列与尖孢刺盘孢Colletotrichum acutatum(有性阶段为尖孢小丛壳Glomerella acutata)的相似性达99%,结合培养性状的观察及rDNA ITS序列分析,确定其为尖孢刺盘孢(C.acutatum),是四川省内首次报道C.acutatum危害辣椒果实的。以该菌为菌源,对60份辣椒材料进行了抗病性鉴定,结果显示所有的材料中仅有4份抗病,14份耐病,剩余的均是感病材料,未发现高抗和免疫的材料。A serious anthracnose disease was found in fruits of chili pepper in Xichong County,Sichuan.It caused severe losses in the fields.The pathogen can infect green and mature pepper fruits,but not infect the blade or the stem in the fields.Lesion on fruit were circular,long oval or irregular.In the laboratory,colonies on PSA was circular,edge tidy,pink or orange,aerial mycelia white,dense,cottony without visible conidial masses,sclerotia absent,setae absent,acervuli absent in culture.Conidia were single-celled,shuttle line and the conidia is 11.703-13.657 μm×3.074-4.773 μm in size.Its appressorium was circular or elliptic,brown,3.985-6.633 μ m×3.954-6.170 μ m in size.The isolated＇s PCR product sequenced by BLAST analysis showed that,its rDNA ITS had 99 % similarity with that of Colletotrichum acutatum（the sexual stage was Glomerella acutata）.The pathogen causing anthracnose of pepper in Xichong was identified as C.acutatum,combining to observation of the culture characters.C.acutatum on chili pepper was reported for the first in Sichuan.The resistance identification result showed that,in all the tested materials（60 varieties or materials in total）,4 varieties or materials were resistant,14 varieties or materials were tolerance,and the others were susceptible materials,and no highly resistant and immune materials.

关 键 词：辣椒 尖孢刺盘孢(Colletotrichum acutatum) 抗病性鉴定 

分 类 号：S436.418.19[农业科学—农业昆虫与害虫防治]