凡纳滨对虾eIF5A基因的克隆及表达分析  

Cloning and Expression Analysis of eIF5AGene from Litopenaeus vannamei

作　　者：马宁[1] 曾地刚[1] 

机构地区：[1]广西水产研究所遗传育种与健康养殖重点实验室,广西南宁530021

出　　处：《西南农业学报》2013年第3期1273-1277,共5页Southwest China Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(31160531)

摘　　要：真核细胞翻译起始因子5A(eIF5A)是真核生物细胞内的蛋白质翻译起始因子。近年来的研究发现eIF5A在动植物细胞衰老死亡、环境胁迫应答和免疫应答等过程中起着重要的调控作用。通过对凡纳滨对虾cDNA文库进行测序,首次获得了包含eIF5A完整的氨基酸序列的cDNA。该cDNA包含474 bp的开放阅读框,编码157个氨基酸,预测分子量约为17.257 ku,理论等电点为5.06。凡纳滨对虾eIF5A和其他物种对比结果显示,该基因的氨基酸序列和其他物种有较高的同源性。实时定量RT-PCR结果显示,eIF5A基因在凡纳滨对虾不同组织中的mRNA表达没有明显的差异。对WSSV、TSV和IHHNV感染的凡纳滨对虾的肝胰腺的实时定量RT-PCR结果显示,病毒感染的凡纳滨对虾的eIF5A基因的表达明显增加,分别是对照样品表达量的2.2、2.5和1.6倍,表明eIF5A可能涉及凡纳滨对虾的抗病毒免疫应答。Eukaryotic translation initiation factor 5A（eIF5A） is a protein translation initiation factor in eukaryotic cells.Recent studies found that eIF5A plays an important role in regulating the processes of cell death,environmental stress response and immune response in animal and plant cells.In the present study,a cDNA containing the complete amino acid sequence of eIF5A was obtained for the first time by sequencing the Litopenaeus vannamei cDNA library.This cDNA contained a 474bp open reading frame encoding 157 amino acids,with the predicted molecular weight of about 17.257ku,and calculated isoelectric point of 5.06.Comparison analysis showed that the amino acid sequence of the L.vannamei eIF5A had high homology with those of other species.Real-time quantitative RT-PCR results indicated that the mRNA expression of eIF5A gene in different tissues of L.vannamei exhibited no significant difference.Real-time quantitative RT-PCR analysis showed that the mRNA levels of eIF5A gene were significantly increased by 2.2-,2.5-and 1.6-fold in the WSSV-,TSV-and IHHNV-infected hepatopancreas of L.vannamei,respectively,as compared to the control,indicating that eIF5A may be involved in the antiviral immune response of L.vannamei.

关 键 词：凡纳滨对虾 真核细胞翻译起始因子5A 克隆 表达 

分 类 号：S945.1[农业科学—水产养殖]