颈交感神经阻滞对严重烧伤大鼠结肠肌细胞表型转化的影响  

Study on the roles of cervical sympathetic block in phenotype switch of the colonic SMCs of rats with burn injury

作　　者：矫立仁[1,2] 谢有富[1,2] 刘志勇[1,2] 童仁联[1,2] 张旭辉[1,2] 

机构地区：[1]暨南大学医学院第四附属医院 [2]广州市红十字会医院烧伤整形科,广东广州510220

出　　处：《长江大学学报（自科版）（下旬）》2013年第7期1-3,18,共4页Journal of Yangtze University

基　　金：广东省科技厅科技计划项目资助(2011B031800310)

摘　　要：目的:探讨颈交感神经阻滞(cervical sympathetic block,CSB)对严重烧伤大鼠的治疗作用,明确颈交感神经阻滞对严重烧伤大鼠胃肠运动功能及结肠平滑肌细胞表型转化的影响。方法:将体质量约200g的SD大鼠随即分成正常组、烧伤对照组和CSB组,烧伤组和CSB组均制作成30%TBSAⅢ度烧伤模型。CSB组通过颈交感神经双侧连续阻滞6d,正常组和对照组颈部连续注射生理盐水6d。通过免疫组化观察大鼠结肠平滑肌α-肌动蛋白表达情况。结果:在实验后的第6天,与正常组相比,烧伤对照组的炭末推进率(61.14%±3.71%vs 76.23%±3.82%)显著降低,而CSB组无明显变化(73.85%±4.10%),但是CSB组炭末推进率要明显高于烧伤对照组。烧伤对照组中,大鼠结肠平滑肌细胞α-肌动蛋白表达水平要显著低于正常组(26.75±3.68vs 35.13±5.27)和CSB组(26.75±3.68vs 32.08±4.53),正常组和CSB组没有明显差异。结论:大鼠的胃肠运动功能在烧伤后明显受到了抑制,通过颈交感神经阻滞能够显著改善这种抑制作用。严重烧伤后的颈交感神经阻滞能够提高结肠肌α-肌动蛋白表达,增强结肠平滑肌的收缩功能,这将有利于肠道运动功能的维持,防止肠道缺血,防止细菌的过度繁殖及移位,促进机体的修复。Objective:To study the effects of cervical sympathetic block(CSB) for the therapy of severe burned rats,explore the roles of cervical sympathetic block in gastrointestinal motility and colonic muscle cell phenotype switch in severe burned rats.Methods:SD rats were randomly divided into Burned and CSB groups.Both of these groups were constructed severe burned model.The TBS Ⅲ burn injure model was used as severe trauma model.Two sides cervical sympathetic block was adopted for 6 days in CSB group.The burned rats in control group were injected with saline for 6 days.Following,observe the expression ofα-actin via immunohistochemisry.Results:In the first six days after the experiment,compared with the normal group(76.23%±3.82%),carbo medicinalis propulsive rate of burned control group(61.14%±3.71%) significantly reduced,while no significant change with the CSB group(73.85%±4.10%).But the carbo medicinalis propulsive rate of CSB group is significantly higher than the burned control.In the burned control group(26.75±3.68),the expression of alpha-smooth muscle actin in the smooth muscle cells of the rat colon is significantly lower than the normal group(35.13±5.27) and CSB group(32.08±4.53),but there is no significant difference between normal group and CSB group.Conclusion:The inhibition of gastrointestinal motility in severe burned rats is significantly improved after cervical sympathetic block.Cervical sympathetic block after severe burns can improve colon muscleα-smooth muscle actin expression which will enhance colonic smooth muscle contraction,benefit the intestinal absorption to prevent bacterial overgrowth and migration,promote the burn repaired.

关 键 词：颈交感神经阻滞 严重烧伤 Α-平滑肌肌动蛋白 胃肠运动 表型转化 

分 类 号：R644[医药卫生—外科学] R365.644[医药卫生—临床医学]