千层塔提取物废液中3,4-二羟基肉桂酸和3,4-二甲氧基肉桂酸的提取  

Extraction of 3,4-Dihydroxy Cinnamic Acid and 3,4-Dimethoxy Cinnamic Acid from Waste Extracted from Huperzia serrata

作　　者：王婷婷[1] 赵丽娟 何山[1] 牟桐[1] 原野[1] 严小军[1] 

机构地区：[1]宁波大学海洋学院,浙江宁波315211 [2]宁波中药制药有限公司,浙江宁波315806

出　　处：《宁波大学学报（理工版）》2013年第3期26-30,共5页Journal of Ningbo University：Natural Science and Engineering Edition

基　　金：浙江省钱江人才计划项目(2012R10068)

摘　　要：利用高速逆流色谱(HSCCC)对千层塔提取物废液中含量较高的2个同系物进行初步纯化分离,再利用高效液相色谱(HPLC)对收集到的样品进行同系物分离,得到8mg化合物A,7mg化合物B,纯度均在95%以上.经核磁共振仪(NMR)鉴定2个组分的化学结构,结果证明,千层塔提取物废液所含的主要成分即2个同系物分别为3,4-二羟基肉桂酸和3,4-二甲氧基肉桂酸.结论也同时证实高速逆流色谱与高效液相色谱联用能够有效分离样品所含同系物,且工业提取千层塔有效成分后的废液中仍含有价值物质.High-speed counter-current chromatography（HSCCC） is first used preliminarily to purify two homologues with higher content in waste from extracts of Huperzia serrata,and high performance liquid chromatography（HPLC） is used to seperate the two components.8 mg compound A and 7 mg compound B are obtained,and purity of both compounds reaches more than 95%.Then chemical structure of the two components is identified using the nuclear magnetic resonance apparatus（NMR）.The results show that the two components are 3,4-dihydroxy cinnamic acid and 3,4-dimethoxy cinnamic acid.Conclusion can be made that the homologues can be purified by combination of high-speed counter-current chromatography and high performance liquid chromatography,and there still are valuable substances in waste from extracts of Huperzia serrata.

关 键 词：高速逆流色谱 高效液相色谱 千层塔 废液 

分 类 号：O652.1[理学—分析化学]