6-BA和AgNO_3对芥菜型油菜下胚轴芽再生的影响  

Effects of 6-BA and AgNO_3 on Shoot Regeneration of Brassica juncea(L.) Hypocotyls

作　　者：袁玉辉[1] 杨柳[1] 刘显军[1] 陆赢[1] 刘忠松[1] 

机构地区：[1]湖南农业大学油料作物研究所/国家油料作物改良中心,长沙410128

出　　处：《作物研究》2013年第2期121-124,共4页Crop Research

基　　金：国家自然科学基金项目(31101176)

摘　　要：以芥菜型油菜品种Brown mustard的下胚轴为外植体,接种于不同配比6-苄基腺嘌呤(6-BA)和硝酸银(AgNO3)组合的分化培养基中,研究6-BA和AgNO3对芥菜型油菜下胚轴芽再生的影响。结果表明分化培养基中含3 mg/L 6-BA+5 mg/L AgNO3时出芽率最高,出芽率达到58.33%,芽丛数平均为1.73。In order to study the effects of 6 -BA and AgNO3 on shoot regeneration of Brassica juncea（ L. ） hypocotyls, we used the Brown mustard （Brassica juncea L. ） hypocotyls as explant, which inoculated in differentiation medium of different concentration ratios of 6 - benzyl adenine （ 6 - BA） and silver nitrate （ AgNO3 ） combination. The results revealed that the shoot regeneration rate was the highest when differentiation medium contained 3 mg/L 6 - BA and 5 mg/L AgNO3, the bud rate reached 58. 33% and the average bud number was 1.73.

关 键 词：芥菜型油菜 下胚轴 芽再生 6-BA AGNO3 

分 类 号：S565.4[农业科学—作物学]