新生大鼠心脏微血管内皮细胞原代培养  

Primary Culture of Cardiac Microvascular Endothelial Cells in Neonatal Rats

作　　者：张滕[1] 郭利平[1] 

机构地区：[1]天津中医药大学

出　　处：《中西医结合心脑血管病杂志》2013年第7期839-840,共2页Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease

基　　金：国家自然基金资助项目(No.81072965)

摘　　要：目的建立乳鼠心脏微血管内皮细胞(CMEC)体外培养研究模型。方法采用混合酶(0.06%胰蛋白酶和0.1%Ⅱ型胶原酶均量混合)消化法和差速贴壁法纯化细胞,倒置显微镜对所培养的细胞进行形态学观察,以Ⅷ因子相关抗原免疫组化染色法进行细胞鉴定。结果通过形态学特征及微血管内皮细胞相关特异性抗原检测证实,得到高纯度乳鼠心脏微血管内皮细胞。结论利用混合酶消化法成功分离并培养乳鼠的CMEC,该方法简单易行,可用于进一步的科学研究。Objective To establish the model in vitro of cardiac microvascular endothelial cells （CMEC） in neonatal rats. Methods CMEC were isolated and purified in hybrid enzyme（0.06% trypsin and 0. 1% Ⅱ collagenase） digestion,different centrifugal force and different adherence, then cultured. The cultured cells morphology was observed by inverted microscope. CMEC were identified by immunohistochemical staining for factor Ⅷ related antigen. Results CMEC were confirmed by morphological characteristics and CMEC associated antigen testing. High purity CMEC were obtained. Conclusion CMEC of neonatal rats were successfully isolated and cultured by hybrid enzyme digestion. The method is simple, can be used for further scientific research.

关 键 词：心脏微血管内皮细胞 原代培养 乳鼠 

分 类 号：R322.1[医药卫生—人体解剖和组织胚胎学] R285.5[医药卫生—基础医学]