肿瘤坏死因子受体相关因子6在小胶质细胞炎性激活中的作用  

The effect of TRAF6 in microglia inflammatory activation

作　　者：孙驰[1] 陶涛[1] 

机构地区：[1]南通大学医学院,江苏226001

出　　处：《交通医学》2013年第3期221-223,共3页Medical Journal of Communications

基　　金：国家自然科学基金资助项目(81202368)

摘　　要：目的:研究肿瘤坏死因子受体相关因子6(TRAF6)在小胶质细胞炎性激活中的作用。方法:分离培养大鼠原代小胶质细胞,利用脂多糖(LPS)处理构建炎症模型,免疫印迹检测TRAF6的表达改变;合成TRAF6的siRNA,转染小胶质细胞后构建LPS诱导的细胞炎性激活模型,检测细胞活化标记F4/80的表达。结果:脂多糖可诱导小胶质细胞中F4/80的表达,并存在浓度依赖性。干预细胞中TRAF6的表达抑制小胶质细胞中活化标记F4/80的表达。结论:TRAF6在小胶质细胞炎性活化过程中表达增加,干预TRAF6的表达抑制细胞炎性活化。Objective：To investigate the role of tumor necrosisfactor receptor-associated factor 6（TRAF 6） in the inflammatory activation of microglia.Methods： Primary rat microglia were isolated and cultured,and inflammation models were built with Lipopolysaccharides（LPS）.Western Blot was used to exam the expression of TRAF6 induced by LPS in microglia.Furthermore,siRNA was used to knockdown TRAF6 expression and Western Blot was used to ananlyze the fuction of TRAF6 in microglia activation maker F4/80.Results： Primary microglia were exposed to LPS,the expression of TRAF6 was up-regulated in dose dependent manner.Knockdown TRAF6 expression could supress microglia activation.Conclusion： In LPS inducded microglia,the expression of TRAF6 was increased.Knockdown TRAF6 expression could supress microglia inflammatory activation.

关 键 词：肿瘤坏死因子受体相关因子 小胶质细胞 脂多糖 WESTERN BLOT检测 大鼠 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]