基于马达蛋白探讨推拿对坐骨神经损伤大鼠轴浆运输功能的影响  

Study on the Influence of Massage to Sciatic Nerve Injured Rat's Axoplasmic Transport Function Based on Motor Protein

作　　者：姚斌彬[1] 梅旭晖[1] 吴剑聪[1] 于天源[1] 

机构地区：[1]北京中医药大学针灸推拿学院,北京100029

出　　处：《南京中医药大学学报》2013年第4期338-341,共4页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：国家自然科学基金(30873311)

摘　　要：目的观察推拿对坐骨神经损伤大鼠轴浆运输马达蛋白表达改变的影响,探讨推拿治疗坐骨神经损伤的起效机制。方法建立大鼠坐骨神经夹持损伤模型,通过腓肠肌湿质量测量、热痛阈实验观察正常组、假手术组、模型组、模型对照组、推拿组大鼠的病理学、行为学变化;通过免疫组织化学染色观察各组大鼠脊髓内kinesin及dynein表达情况,并统计分析各组间的差异。结果通过对模型组和模型对照组大鼠热痛阈、腓肠肌湿质量的检测,得出坐骨神经损伤后大鼠的感觉及运动功能明显降低的结果,在推拿治疗后,腓肠肌湿质量测量及热痛阈实验评分明显升高,并在治疗20d后与正常组无显著性差异;模型组、对照组及推拿组kinesin及dynein免疫组化表达与正常组相比均有显著性提高,推拿组与模型组、对照组相比也具有显著性差异。结论推拿治疗可以通过提高轴浆运输马达蛋白kinesin及dynein的表达,促进轴浆运输功能恢复,进而双向影响神经元细胞及神经支配靶组织,并最终改善坐骨神经损伤大鼠的运动及感觉功能。OBJECTIVE To observe the influence of massage to the express change of sciatic nerve injured rat＇s axoplasmic transporting motor protein.To investigate the effect mechanism of massage treating sciatic nerve injury.METHODS A sciatic nerve clamping rat model was set.The pathological and behavioral changes of rats in normal group,sham operation group,model group,model control group and massage group were observed through measuring the wet weight of the gastrocnemius and heat pain threshold experiment.Kinesin in rats＇ spinal cord and dynein express situation were observed by immunohistochemical staining and the differences between each group were counted.RESULTS The rat＇s sense and movement function decreased obviously after its sciatic nerve was injured according to the heat pain threshold and wet weight of the gastrocnemius tests of rats in the model group and model control group.After the massage treatment,the experimental evaluation of wet weight of the gastrocnemius test and heat pain threshold experiment increase significantly and there were no significant difference as compared with the normal group 20 days after treatment.The kinesin and dynein immunohistochemical expression of the model group,control group and massage group obviously increased compared with the normal group.There were significant differences among massage group,model group and control group.CONCLUSION The massage treatment can promote the recovery of axoplasmic transporting function by increasing the expression of axoplasmic transporting motor protein kinesin and dynein.Then the neuronal cells and nerve innervation targeted tissue are two-way influenced.Finally,massage treatment can improve the sense and movement function of sciatic nerve injured rat.

关 键 词：推拿 坐骨神经损伤 轴浆运输 马达蛋白 

分 类 号：R285.5[医药卫生—中药学]