川芎水提部位化学成分的UPLC?ESI?Q?TOF?MS分析  

Analysis on Chemical Components from Water Extract of Ligusticum Chuanxiong by UPLC-ESI-Q-TOF-MS

作　　者：徐晓芳[1,2] 孙东东[2] 李祥[2] 陈建伟[2] 崔九成[1] 

机构地区：[1]陕西中医学院药学院,陕西咸阳712046 [2]南京中医药大学药学院,江苏南京210023

出　　处：《南京中医药大学学报》2013年第4期382-386,共5页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：江苏高校优势学科建设工程资助项目(2011ZYX6-014);江苏省2012年度普通高校研究生科研创新计划项目(CXZZ12-0626)

摘　　要：目的采用UPLC-ESI-Q-TOF-MS技术对川芎水提取部分中的化学成分进行定性分析。方法采用LC-MS/MS,C18色谱柱(4.6mm×250mm,5μm)以甲醇-0.1%甲酸溶液为流动相梯度洗脱;质谱使用ESI离子源,正离子与负离子模式下采集数据。结果通过正、负离子质谱信息及元素组成,分析与相关文献数据对照,共鉴定出13个化合物。结论通过超高效液相色谱分离,质谱测定相对分子质量及相关文献数据信息检索确定川芎水提取部分中的化学成分,为阐明川芎水提部分的药效物质基础提供有力的证据。OBJECTIVE To make a qualitative analysis on chemical components in water extract of Ligusticum chuanxiong by UPLC-ESI-Q-TOF-MS.METHODS LC-MS/MS,C18 （4.6 mm× 250 mm,5 μm） column was adopted,with methanol-0.1 ％ formic acid as the mobile phase for gradient elution; ESI ion source was used for mass spectra,and data were collected in positive and negative mode.RESULTS By analyzing positive and negative ion mass spectra information,element composition as well as comparing controls with data from relevant literature,thirteen compounds from water extract of Ligusticum chuanxiong Hort were identified.CONCLUSION After the separation by ultra high performance liquid chromatography,relative molecular mass was determined by mass spectra,and chemical compounds in water extract of Ligusticum chuanxiong Hort were determined by information search through relevant literature data,which served as a powerful proof for pharmacodynamic material basis of water extract from Ligusticum chuanxiong.

关 键 词：川芎 化学成分 UPLC-ESI-Q-TOF-MS 

分 类 号：R284.1[医药卫生—中药学]