低强度红激光局部照射抑制血管成形术后血管重塑的可能机制  

The potential mechanisms of LPRLI attenuates the constrictive vascular remodeling after balloon injury in atherosclerotic rabbits

作　　者：耿庆山[1] 冯建章[1] 陈纪言[1] 周颖玲[1] 李光[1] 陈颜芳[1] 林秋雄[1] 

机构地区：[1]广东省心血管病研究所心内科,广州市510100

出　　处：《岭南心血管病杂志》2000年第3期195-198,171,共5页South China Journal of Cardiovascular Diseases

摘　　要：目的 观察低强度红激光照射（Ｌｏｗ Ｐｏｗｅｒ ＲｅｄＬａｓｅｒ　Ｉｒｒａｄｉａｔｉｏｎ，ＬＰＲＬＩ）后局部血管组织Ⅰ型胶原（Ｃｏｌ　Ⅰ）ｍＲＮＡ、细胞间黏附分子－１（ＩＣＡＭ－１）的表达和血管平滑肌细胞（ＶＳＭＣ）凋亡，探讨ＬＰＲＬＩ抑制动脉粥样硬化兔血管成形术后收缩性血管重塑的可能机制。方法 建立动脉粥样硬化兔的血管损伤模型。１００只新西兰白兔随机分为单纯血管拉伤组和单纯拉伤＋激光照射组。对术后３天、１周、２周、４周、８周动物组织提取总 ＲＮＡ、半定量 ＲＴ－ＰＣＲ观察 Ｃｏｌ ｍＲ－ＮＡ表达和免疫组织化学－ＬＳＡＢ法测定ＩＣＡＭ－１、ｐ５３、ｂｃｌ－２／ｂａｘ。ＴＵＮＥＬ法检测ＶＳＭＣ凋亡并计算凋亡指数。结果①Ｃｏｌ　Ⅰ　ｍＲＮＡ的表达：术后３天，两组均可见到Ｃｏｌ　Ⅰ　ｍＲＮＡ的表达，但无统计学意义；术后第 １～４周，Ｃｏｌ　Ⅰ ｍＲＮＡ的表达ＬＰＲＬＩ组明显低于对照组；第８周，两组Ｃｏｌ　Ⅰ　ｍＲＮＡ的表达均减弱，组间无统计学差异。②ＩＣＡＭ－１的表达：免疫组织化学检测术后３天即可看到ＩＣＡＭ－１的表达，ＬＰＲＬＩ组明显低于对照组；第１周到第４周，ＬＰＲＬＩ组的ＩＣＡＭ－１虽然逐渐增强，但仍然显著低于对照组?Objective In this study, we investi-gated whether LPRLI (Low Power Red Laser Irradiation) could attenuate the expressions of Col I mRNA, ICAM-1, p53, bcl-2/bax and induce apoptosis of VSMC in the rabbit abdominal aorta injury model. Methods We estab-lished atherosclerotic arterial stenosis models successfully.100 male New Zealand white rabbits were randomized into two groups: balloon injury group and balloon injury plus laser irradiation group (total irradiation density: 0. 9J/ cm2). After 3 days, 1 week, 2 weeks, 4 weeks, 8 weeks, abdominal aortas were harvested for reverse tran-scription-polymerase chain reaction (RT-PCR) studies and immunohistocheical studies on the expressions of Col I mRNA, ICAM-1, p53,bcl-2/bax. The presence of apoptotic VSMC was demonstrated by terminal deoxynucleotidyl transferase-mediated dUTP nickl end labeling (TUNEL) method. Results (1)The expression of Col I mRNA at 3rd day after balloon injury, the two groups (LPRLI group vs control group) did not show a significantly difference [(114*24) cmp,vs (118* 11)cmp, P >0.05]. from 1st week to 4th week, the expressions of Col I mRNA showed a significantly difference. The den-sitometric data of RT-PCR in LPRLL group was lower than the control group [(760* 119) cpm vs (879* 121) cpm, P<0.05;(1 120*300) cpm vs (1409*297) cpm, P <0.05; (918 * 180) cpm vs (1109 * 117) cpm, P <0.05];(2)The expression of ICAM-1 from 3rd day to 4th week, the expressions of ICAM- 1 in LPRLI group was significantly reduced both at the intima and ad- ventitia[(0.90*0.48) vs (1.79*0.91), P<0.05; (1.41*0.89) vs (3.46*1.57), P<0.01; (2.60* 0.91) vs (3.51*0.99), P<0.05;(2.31*1.00)vs (2.57 * 1.20), P < 0.05](3)Apoptosis of VSMC. These studies suggested that there were histochemical evidence of apoptosis in all of the injured vessels. There were no quantitative differences in the amount of LPRLI and control vessels at 3 days, 1 week and 8 weeks. But there were marked differences between the two groups. The index of apoptotic VSMC in LPRLI group was higher than control group

关 键 词：激光 血管重塑 凋亡 平滑肌细胞 

分 类 号：R654.3[医药卫生—外科学]