舟山群岛野生山茶种群遗传多样性的ISSR分析  

Analysis on Genetic Diversity of Camellia japonica in Zhoushan Archipelago by ISSR Markers

作　　者：林立[1] 胡仲义[1] 王国明 

机构地区：[1]宁波城市职业技术学院,宁波315502 [2]浙江省舟山市林业科学研究所,定海316000

出　　处：《植物遗传资源学报》2013年第4期594-599,共6页Journal of Plant Genetic Resources

基　　金：浙江省科技厅重点项目(2008C14065)

摘　　要：采用ISSR分子标记,利用筛选的20条引物对舟山群岛4个野生山茶(Camellia japonica)种群的遗传多样性进行分析。结果表明:山茶种群的多态性位点百分比(PPB)为64.06%,Nei's基因多样性指数(He)为0.2390,Shannon信息多态性指数(H)为0.3548,种群水平遗传多样性较高。基因分化系数Gst=0.2241,表明种群间具有较高的遗传分化。地理距离与遗传距离具有显著相关性(r=0.9653,P<0.05),表明岛屿隔离对山茶种群的遗传分化具有重要影响。UPGMA聚类表明同岛种群间的亲缘关系更近。基于舟山群岛山茶种群遗传结构分析,建议在其自然生长地加强就地保护。Camellia japonica is an important horticultural species,widely distributed in East-Asia.Four populations of C.japonica were analyzed by ISSR markers to detect the genetic diversity.Twenty primers with discernible DNA bands markers were applied.The percentage of polymorphic bands,Nei’s gene diversity,and Shannon’s information index were 64.06%,0.2390,and 0.3548,respectively,indicated that the genetic diversity of C.japonica populations was relatively high.The value of differentiation（Gst = 0.2241） affirmed that C.japonica had high genetic differentiation among populations.The effect of geographical isolation had great influences and significant correlation was found between genetic distance and geographic distance（r = 0.9653,P ＆lt; 0.05）.UPGMA analysis revealed that populations on the same island had a closer relationship.In order to maintain genetic diversity and resource utilization of C.japonica,management strategies were proposed that natural population should be protected in situ from human disturbance to facilitate its natural generation.

关 键 词：山茶 ISSR 舟山群岛 遗传多样性 

分 类 号：S685.14[农业科学—观赏园艺]