大粒裸燕麦(Avena nuda L.)遗传连锁图谱的构建  

A Genetic Linkage Map for Naked Oat (Avena nuda L.)

作　　者：徐微[1,2] 张宗文[1,3] 张恩来[1,4] 吴斌[1] 

机构地区：[1]中国农业科学院作物科学研究所,北京100081 [2]嘉兴学院生物与化学工程学院,嘉兴314001 [3]国际生物多样性中心东亚办事处,北京100081 [4]云南省农业科学院生物技术与种质资源研究所,昆明650223

出　　处：《植物遗传资源学报》2013年第4期673-678,共6页Journal of Plant Genetic Resources

基　　金：国家科技支撑计划项目(2006BAD02B05-11);国家燕麦产业技术体系(nycytx-14)

摘　　要：以元莜麦和555杂交得到的281个F2单株为作图群体,利用20对AFLP引物、3对SSR引物和1个穗型性状构建了一张大粒裸燕麦遗传连锁图。该图谱全长1544.8 cM,包含19个连锁群,其上分布有92个AFLP标记、3个SSR标记和1个穗型形态标记,不同连锁群标记数为2～14个,长度在23.7～276.3 cM之间,平均长度为81.3 cM,标记间平均距离为20.1 cM。穗型标记分离比符合3∶1,11个AFLP标记表现为偏分离,偏分离比为11.5%。该图谱符合遗传连锁框架图的要求,为今后大粒裸燕麦的QTL定位、分子标记辅助育种和比较基因组学等研究奠定基础。Based on 281 individual plants of F2 population derived from a cross＂Yuan Naked Oat＂x＂555＂,a genetic linkage map for naked oat（Avena nuda L.） was constructed by 20 AFLP primer pairs,3 SSR primer pairs,and 1 panicle type character.The map was 1544.8 cM in total length with 20.1 cM for the average distance between neighboring markers.92 AFLP markers,3 SSR markers,and 1 morphological trait were mapped on 19 linkage groups,which contained 2-14 markers and varied in size from 23.7 cM to 276.3 cM with an average of 81.3 cM.The segregation ratio of panicle type fitted to 3∶ 1,and 11 AFLP markers demonstrated distorted segregation with the percentage of 11.5%.The results provided a framework of genetic linkage map for naked oat（Avena nuda L.）,which was the theoretical basis for QTL mapping,molecular breeding,and comparative genomics in naked oat research.

关 键 词：大粒裸燕麦 遗传连锁图谱 AFLP SSR 

分 类 号：S512.6[农业科学—作物学]