大白菜相对于结球甘蓝连锁群特异SSR标记的筛选  

Screening of Specific SSR Markers on Different Linkage Groups of Chinese Cabbage Compared with Cabbage

作　　者：李雪姣[1,2] 王彦华[1] 轩淑欣[1] 赵建军[1] 顾爱侠[1] 

机构地区：[1]河北农业大学园艺学院,保定071001 [2]河北省邢台市农业局,邢台054001

出　　处：《植物遗传资源学报》2013年第4期694-698,共5页Journal of Plant Genetic Resources

基　　金：国家自然科学基金(31171964);河北省自然科学基金(C2010000677)

摘　　要：以9个大白菜品种和10个结球甘蓝品种为试材,对位于芸薹属A基因组10个连锁群的207对SSR引物进行PCR筛选,共筛选出33个大白菜相对于结球甘蓝的特异SSR标记,涉及了大白菜的10个连锁群,其中位于A1连锁群3对,A2连锁群4对,A3连锁群5对,A4连锁群2对,A5连锁群3对,A6连锁群4对,A7连锁群2对,A8连锁群1对,A9连锁群2对,A10连锁群7对。为进一步利用SSR标记鉴定结球甘蓝—大白菜异附加系奠定了基础。A total of 207 pairs of SSR primers from 10 linkage groups of Brassica（A genome） were amplified on 9 Chinese cabbage varieties and 10 cabbage varieties.The result showed that 33 SSR markers were specific on Chinese cabbage compared with cabbage.These markers assigned on 10 different linkage groups of Chinese cabbage,in which 3 SSR markers on group A1,4 SSR markers on group A2,5 SSR markers on group A3,2 SSR markers on group A4,3 SSR markers on group A5,4 SSR markers on group A6,2 SSR markers on group A7,1 SSR marker on group A8,2 SSR markers on group A9,and 7 SSR markers on group A10.It laid a foundation to further identify cabbage-Chinese cabbage alien addition lines.

关 键 词：结球甘蓝 大白菜 连锁群 SSR标记 

分 类 号：S634.1[农业科学—蔬菜学] S635.1[农业科学—园艺学]