新型抗除草剂棉花不育系Yu98-8A1的培育及鉴定  

Breeding and Identification of Herbicide-resistant Genic Male-sterile Line Yu98-8A1 of Cotton(Gossypium hirsutum L.)

作　　者：杨晓杰[1] 谢德意[1] 赵元明[1] 李武[1] 赵付安[1] 段铮铮[1] 祝水金[2] 房卫平[1] 

机构地区：[1]河南省农业科学院经济作物研究所,郑州450002 [2]浙江大学农业与生物技术学院,杭州310058

出　　处：《植物遗传资源学报》2013年第4期723-727,共5页Journal of Plant Genetic Resources

基　　金：河南省科技攻关项目(122102110057);国家转基因重大专项(2011ZX08005-005-006);国家"863"项目(2012AA101108-02)

摘　　要：通过连续回交,将抗除草剂基因EPSPS-G6转育花粉败育彻底(无微量花粉,不育度达100%)的棉花单基因隐性控制的核不育系Yu98-8A,进而培育成抗除草剂核不育系Yu98-8A1。对该转育不育系花冠表型测量观察表明,与同质系正常可育株比较,不育株花冠较小,不育株子房直径略大于可育株,花柱长和花柱外露长度均明显高于同质系正常可育株,花柱头外露为其最显著的表型特征;显微观察显示,不育系Yu98-8A1小孢子败育主要是在四分体形成后的小孢子发育期。小孢子败育特征表现为花粉粒无内含物、无刺突产生,最后解体、退化。PCR分子鉴定表明,抗除草剂基因EPSPS-G6转育入Yu98-8A,除草剂抗性试验表明,该转育不育系可抗质量百分比浓度达0.3%的草甘膦。该抗除草剂核不育系的培育在棉花杂种优势利用方面有重大利用价值。Yu98-8A1,a herbicide-resistant cotton genic male-sterile line,was developed from a genic malesterile line Yu98-8A by successive backcrossing cotton line EHC09-002 which possessed the herbicide-resistant gene EPSPS-G6.Compared with homogeneity normal fertile plants,its significant characteristics included smaller corolla,bigger Ovary diameter,style length,and exposed stigmas by corolla phenotype observation.Among theses characteristics,exposed stigmas was the most significant one.Cytological observation showed that the anther abortion of Yu98-8A1 occurred during the stage of microspore development,which displayed without inclusion in pollen grain and spinescent protuberance on the pollen wall,and the microspore appeared abnormal and break down gradually.PCR identification and glyphosate resistance test indicated that herbicide-resistant gene EPSPS-G6 had been successfully aggregated into Yu98-8A,and the anti-herbicide concentration reached up to 0.3%.The cultivation of herbicide-resistant sterile line made Yu98-8A1 have significant value in the utilization of cotton heterosis.

关 键 词：陆地棉 抗除草剂不育系Yu98-8A1 培育及分子鉴定 

分 类 号：S562[农业科学—作物学]