红掌品种亲缘关系SRAP分析  

Analysis of Gnetic Relationships of Anthurium andreanum Varieties Using SRAP Markers

作　　者：王呈丹[1,2] 牛俊海[2] 张志群[2] 潘宏兵[2] 任羽[2] 

机构地区：[1]海南大学农学院,海口570100 [2]中国热带农业科学院热带作物品种资源研究所/农业部华南作物基因资源与种质创制重点实验室,儋州571737

出　　处：《植物遗传资源学报》2013年第4期759-763,共5页Journal of Plant Genetic Resources

基　　金：国家科技支撑计划项目(2007BAD45B06);海南省重大科技研发专项(2009YFZX007);海南省科技成果示范推广专项(CGTG20100004)

摘　　要：利用相关序列扩增多态性(SRAP)分子标记,从100对引物组合中筛选出26对多态性高、条带清晰的SRAP引物,对33个红掌品种进行遗传多样性和亲缘关系分析。结果如下:(1)26对引物共扩增出366条带,其中有314条多态性条带,多态性比率为85.79%。引物组合产生的条带数在9～23之间,平均每对引物组合扩增出14.1条和12.1条多态性条带。(2)根据SRAP扩增结果,利用UPGMA法进行聚类分析,33份材料的遗传相似系数在0.55～0.94之间,在遗传相似系数0.786处可将33个红掌品种分为5个类群。结果表明,供试品种遗传多样性丰富,本研究为品种鉴定和杂交育种提供了参考信息。Sequence-related amplified polymorphism（SRAP） analysis was carried out to evaluate the genetic diversity and genetic relationships among 33 Anthurium andraeanum cultivars.Out of 100 pairs of primers screened twenty-six pairs of SRAP primers were used to analyze genetic diversity of the tested materials.The results showed that：（1） Twenty-six primer pairs amplified out 366 bands,out of which 314 bands were polymorphic bands,the percentage of polymorphic bands was 85.79%.The number of bands ranged from 9 to 23 with an average of 14.1 bands and 12.1 polymorphic bands per primer.（2） According to the SRAP amplification results,the genetic similarity coefficient among tested cultivars was in a range from 0.55 to 0.94,a dendrogram produced using unweighted pair group method using arithmetic averages（UPGMA）,and 33 Anthurium andreanum cultivars were divided into five groups when genetic similarity coefficient was given as 0.786.The Anthurium cultivars showed a high genetic diversity,and the results would facilitate variety identification and cross breeding.

关 键 词：红掌 SRAP 遗传多样性 聚类分析 

分 类 号：S682.14[农业科学—观赏园艺]