椰甲截脉姬小蜂室内外种群过氧化物酶和酯酶同工酶的比较  

Peroxidase and Esterase Isozymes Comparison of Laboratory and Field Populations of Asecodes hispinarum Boucek

作　　者：姜迎娅[1] 龚声辉[1] 朱麟[1] 

机构地区：[1]海南师范大学生命科学学院,海南省热带动植物生态学重点实验室,海南海口571158

出　　处：《海南师范大学学报（自然科学版）》2013年第2期204-207,共4页Journal of Hainan Normal University（Natural Science）

基　　金：国家大学生创新实验计划资助(101165829)

摘　　要：采用不连续垂直平板聚丙烯酰胺凝胶电泳(PAGE),对椰甲截脉姬小蜂的室内和野外种群的过氧化物酶(POD)和酯酶(EST)同工酶分别进行了分析.结果表明:椰甲截脉姬小蜂室内和野外两个种群的POD同工酶和EST同工酶谱带均存在差异,酶带颜色也深浅不同.野外种群与室内种群相比,POD同工酶多一条迁移率为0.038的酶带,EST同工酶多一条迁移率为0.58的酶带.说明椰甲截脉姬小蜂室内种群与野外种群存在较明显的差异.The discontinuous vertical fiat polyacrylamide gel electrophoresis （PAGE） method was applied to compare the peroxidase （POD） and Esterase （EST） isozyme differences between laboratory and field populations of Asecodes hi- spinarum Bouek. The POD and EST isozyme electrophoresis bands differed significantly between the two populations, including band color. One enzyme band in POD isozymes with Rf=0.038 and one band in EST isozymes with R/=0.58 on- ly presented in field population. This indicated that population of Asecodes hispinarum has changed physiologically after released in the field.

关 键 词：椰甲截脉姬小蜂 过氧化物酶同工酶 酯酶同工酶 聚丙烯酰胺凝胶电泳 

分 类 号：Q966[生物学—昆虫学]