甘草酸合成生物学元件初探：甘草法呢基焦磷酸合酶基因的克隆及序列分析  

Development of the Devices for Synthetic Biology of Glycyrrhizin at an Early Stage: Cloning and Sequencing Analysis of Farnesyl-Diphosphate Synthase Genes in Glycyrrhiza uralensis

作　　者：李滢[1] 牛云云[1] 宋经元[1] 罗红梅[1] 李秋实[1] 孙超[1] 

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所,北京100193

出　　处：《世界科学技术-中医药现代化》2013年第3期355-359,共5页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：科学技术部国家科技支撑计划(2012BAI29B01):基于本草基因组的中药材活性成分生合成及育种示范研究,负责人:宋经元

摘　　要：甘草酸是中药甘草的主要有效成分,法呢基焦磷酸合酶(FPS)是甘草酸合成途径中关键酶之一。根据甘草转录组数据设计引物,对FPS基因进行克隆,获得甘草FPS基因的全长编码序列。甘草FPS基因编码区全长1 029 bp,编码342个氨基酸残基。将甘草FPS基因与GenBank中其他植物FPS基因序列进行比对分析,发现其核酸序列与鹰嘴豆、苜蓿、蒺藜苜蓿、大豆、百脉根的序列相似度最高,分别达到94%、94%、93%、93%、92%。进一步的系统进化分析表明FPS基因具有较高的保守性,其序列进化树与植物分类一致。Glycyrrhizin is the major bioactive compound in Glycyrrhiza uralensis.Farnesyl-diphosphate synthase（FPS） is one of the major enzymes involved in the glycyrrhizin biosynthetic.We cloned the full length of FPS coding sequence based on the transcriptome data of G.uralensis.The FPS gene of G.uralensis（GlyurFPS） is 1029 bp,coding 342 amino acid.The homology between the sequence of its protein and that of the FPS in Cicer arietinum,Medicago sativa,Medicago truncatula,Glycine max,Lotus japonicus was of 94%,94%,93%,93% and 92%,respectively.Plant FPS gene is very conservative,therefore the phylogenetic tree is same with the plant classification consistent.

关 键 词：甘草 法呢基焦磷酸合酶 甘草酸 基因克隆 

分 类 号：R282[医药卫生—中药学]